Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study

Background- Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. Methods- In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A- 0.1μg/mL Z+A, Group B- 0.1μg/mL Z without A, Group C- 0.2μg/mL Z+A, Group D- 0.2μg/mL Z without A, Group E- 0.3μg/mL Z+A, Group F- 0.3μg/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P<0.05). Results- At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p<0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p<0.001). Conclusions- Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.