The Effect of TNF-α on the Expression of MMP9 in Human Mesenchymal Bone Marrow-Derived Stem Cells

Matrix metalloproteinase 9 (MMP9) as the enzyme of adult stem cells secreted from damage cells. In spite of low level of MMP9 enzyme in the mesenchymal stem cells, many inflammatory cytokines stimulation such as TNF-α could increase MMP9 level in cells. Current study evaluated the expression of the MMP9 enzyme under the influence of TNF-α in human bone marrow mesenchymal stem cells. The human bone marrow mesenchymal stem cells were classified into control and experimental groups. In the experimental groups, various concentrations of the TNF-α (1ng/ml and 10ng/ml) were administrated in different times (10 and 24 hours), whereas the control group was not treated with TNF-α. MMP9 gene expression was evaluated by Real-Time PCR. TNF-α administration in 1ng/ml and 10ng/ml dosage for 10 hours, induced the expression of MMP9 1468.3 and 1782.8 times more than the control group, respectively. After 24h, in comparison between 1ng/ml and 10ng/ml with control groups, MMP9 expression were 442.64 and 1184.4 times more than control group, respectively. In conclusion, the expression rate of the MMP9 gene in bone marrow mesenchymal stem cells might be effected by dosage and time of exposure to TNF-α. Furthermore, the time of exposure might have the prominent role in alteration of MMP9 gene expression induction in the mesenchymal stem cells.