IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v14y2022i17p11075-d907115.html
   My bibliography  Save this article

Large-Grain and Semidwarf Isogenic Rice Koshihikari Integrated with GW2 and sd1

Author

Listed:
  • Motonori Tomita

    (Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City 422-8529, Shizuoka, Japan)

  • Hideumi Ebata

    (Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City 422-8529, Shizuoka, Japan)

  • Kohei Nakayama

    (Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City 422-8529, Shizuoka, Japan)

Abstract

Intending to contribute to sustainable agriculture by “New Green Revolution,” we developed a large-grain/semidwarf isogenic line “Koshihikari sd1GW2” that incorporates both the large-grain gene GW2 and semidwarf gene sd1 . GW2 homozygous B 3 F 2 plant with the genomic background of Koshihikari was backcrossed twice with “Koshihikari sd1.” Koshihikari sd1GW2 fixed in BC 5 F 3 was found to be 12.6 cm shorter than Koshihikari. Whole-genome sequencing proved one deletion in GW2 at 8,147,416 bp on chromosome 2 and the SNPs in sd1 at 38,267,510 bp on chromosome 1. The size of the DNA fragments integrated with each gene was determined as the distance between both ends of SNP clusters. Through the backcrossing from BC 4 to BC 5 , the DNA fragment integrated with GW2 decreased by 148,139 bp. The thousand-grain weight of Koshihikari sd1GW2 (27.8 g) was 18% greater than that of Koshihikari (23.6 g), and the grain yield of Koshihikari sd1GW2 (42.6 kg/a) was 0.5% higher than that of Koshihikari (42.4 kg/a). Our results suggested that Koshihikari sd1GW2 will be less susceptible to lodging by typhoons, cyclones, and heavy rainfall, ordinarily a concern in heavier panicle weight cultivars. We successfully integrated GW2 with sd1 for the first time, specifically in the genome of the leading, globally produced Japonica cultivar Koshihikari.

Suggested Citation

  • Motonori Tomita & Hideumi Ebata & Kohei Nakayama, 2022. "Large-Grain and Semidwarf Isogenic Rice Koshihikari Integrated with GW2 and sd1," Sustainability, MDPI, vol. 14(17), pages 1-15, September.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:17:p:11075-:d:907115
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/14/17/11075/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/14/17/11075/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Youlin Peng & Yungao Hu & Qian Qian & Deyong Ren, 2021. "Progress and Prospect of Breeding Utilization of Green Revolution Gene SD 1 in Rice," Agriculture, MDPI, vol. 11(7), pages 1-11, June.
    2. A. Sasaki & M. Ashikari & M. Ueguchi-Tanaka & H. Itoh & A. Nishimura & D. Swapan & K. Ishiyama & T. Saito & M. Kobayashi & G. S. Khush & H. Kitano & M. Matsuoka, 2002. "A mutant gibberellin-synthesis gene in rice," Nature, Nature, vol. 416(6882), pages 701-702, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Wenlong Yang & Ameer Ahmed Mirbahar & Muhammad Shoaib & Xueyuan Lou & Linhe Sun & Jiazhu Sun & Kehui Zhan & Aimin Zhang, 2022. "The Carotenoid Cleavage Dioxygenase Gene CCD7-B , at Large, Is Associated with Tillering in Common Wheat," Agriculture, MDPI, vol. 12(2), pages 1-14, February.
    2. Hai-Yan Lü & Xiao-Fen Liu & Shi-Ping Wei & Yuan-Ming Zhang, 2011. "Epistatic Association Mapping in Homozygous Crop Cultivars," PLOS ONE, Public Library of Science, vol. 6(3), pages 1-10, March.
    3. Bennett, J., 2003. "Opportunities for increasing water productivity of CGIAR crops through plant breeding and molecular biology," IWMI Books, Reports H032638, International Water Management Institute.
    4. Yakun Wang & Shengjia Tang & Naihui Guo & Ruihu An & Zongliang Ren & Shikai Hu & Xiangjin Wei & Guiai Jiao & Lihong Xie & Ling Wang & Ying Chen & Fengli Zhao & Peisong Hu & Zhonghua Sheng & Shaoqing T, 2023. "Base Editing of EUI1 Improves the Elongation of the Uppermost Internode in Two-Line Male Sterile Rice Lines," Agriculture, MDPI, vol. 13(3), pages 1-13, March.
    5. Isnaini Isnaini & Yudhistira Nugraha & Niranjan Baisakh & Nono Carsono, 2023. "Toward Food Security in 2050: Gene Pyramiding for Climate-Smart Rice," Sustainability, MDPI, vol. 15(19), pages 1-35, September.
    6. Huali Yang & Qinqin Yang & Yiwei Kang & Miao Zhang & Xiaodeng Zhan & Liyong Cao & Shihua Cheng & Weixun Wu & Yingxin Zhang, 2022. "Finding Stable QTL for Plant Height in Super Hybrid Rice," Agriculture, MDPI, vol. 12(2), pages 1-10, January.
    7. Daisuke Ogawa & Toshihiro Sakamoto & Hiroshi Tsunematsu & Toshio Yamamoto & Noriko Kanno & Yasunori Nonoue & Jun-ichi Yonemaru, 2019. "Surveillance of panicle positions by unmanned aerial vehicle to reveal morphological features of rice," PLOS ONE, Public Library of Science, vol. 14(10), pages 1-13, October.
    8. Youlin Peng & Yungao Hu & Qian Qian & Deyong Ren, 2021. "Progress and Prospect of Breeding Utilization of Green Revolution Gene SD 1 in Rice," Agriculture, MDPI, vol. 11(7), pages 1-11, June.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:14:y:2022:i:17:p:11075-:d:907115. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.