IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v12y2020i19p8252-d424567.html
   My bibliography  Save this article

MPN Drop Agar Method for Determination of Heterotrophic Microorganisms in Soil and Water Samples Using Tissue Plate as a Carrier

Author

Listed:
  • Jana Chumchalová

    (Department of Environmental Chemistry, Faculty of Environmental Technology, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic)

  • Martin Kubal

    (Department of Environmental Chemistry, Faculty of Environmental Technology, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic)

Abstract

The most probable number (MPN) method is a culture-based method commonly used in the field of environmental microbiology to examine microbial populations in liquid substrates. The MPN tests require a wide range of special laboratory equipment, a lot of laboratory space, and skilled staff, which together limit their applicability. This paper presents a modified MPN method, which reduces the experimental requirements by applying tissue plate as a carrier. The modified MPN method introduces a fast-filled tissue plate with 5 × 5 squares as agar carrier, instead of the commonly used set of glass tubes. Further, self-refilling automatic syringe Socorex ® was implemented to apply samples to the plate. The response of the modified MPN method was tested on eight selected bacterial strains as well as on soil and water samples. Simultaneously, all the strains and samples were tested by standard spread plate method. High linear correlation between the two methods was found, which makes a new modified MPN method a useful alternative within the field of environmental microbiology.

Suggested Citation

  • Jana Chumchalová & Martin Kubal, 2020. "MPN Drop Agar Method for Determination of Heterotrophic Microorganisms in Soil and Water Samples Using Tissue Plate as a Carrier," Sustainability, MDPI, vol. 12(19), pages 1-8, October.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:19:p:8252-:d:424567
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/12/19/8252/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/12/19/8252/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Karolina Furtak & Jarosław Grządziel & Anna Gałązka & Jacek Niedźwiecki, 2019. "Analysis of Soil Properties, Bacterial Community Composition, and Metabolic Diversity in Fluvisols of a Floodplain Area," Sustainability, MDPI, vol. 11(14), pages 1-20, July.
    2. Bryan S. Griffiths & Jack Faber & Jaap Bloem, 2018. "Applying Soil Health Indicators to Encourage Sustainable Soil Use: The Transition from Scientific Study to Practical Application," Sustainability, MDPI, vol. 10(9), pages 1-14, August.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhihong Zhang & Yali Wu & Vi Khanh Truong & Dongguang Zhang, 2021. "Earthworm ( Eisenia fetida ) Mucus Inspired Bionic Fertilizer to Stimulate Maize ( Zea mays L.) Growth," Sustainability, MDPI, vol. 13(8), pages 1-21, April.
    2. Toru Hamamoto & Yoshitaka Uchida, 2019. "The Role of Different Earthworm Species ( Metaphire Hilgendorfi and Eisenia Fetida ) on CO 2 Emissions and Microbial Biomass during Barley Decomposition," Sustainability, MDPI, vol. 11(23), pages 1-10, November.
    3. Abbas Ali Abid & Xiang Zou & Longda Gong & Antonio Castellano-Hinojosa & Muhammad Afzal & Hongjie Di & Qichun Zhang, 2022. "Physicochemical Variables Better Explain Changes in Microbial Community Structure and Abundance under Alternate Wetting and Drying Events," Agriculture, MDPI, vol. 12(6), pages 1-13, May.
    4. Johannes Rüdisser & Erich Tasser & Thomas Peham & Erwin Meyer & Ulrike Tappeiner, 2020. "Hidden Engineers and Service Providers: Earthworms in Agricultural Land-Use Types of South Tyrol, Italy," Sustainability, MDPI, vol. 13(1), pages 1-14, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:12:y:2020:i:19:p:8252-:d:424567. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.