IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v19y2022i9p5773-d811718.html
   My bibliography  Save this article

Analysis of Environmental and Pathogenic Bacteria Attached to Aerosol Particles Size-Separated with a Metal Mesh Device

Author

Listed:
  • Xiaobo Yin

    (Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama 526-0829, Japan)

  • Seiji Kamba

    (Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama 526-0829, Japan)

  • Koki Yamamoto

    (Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama 526-0829, Japan)

  • Atsushi Ogura

    (Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama 526-0829, Japan)

  • Ernest Apondi Wandera

    (Kenya Research Station, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan)

  • Mohammad Monir Shah

    (Department of Pediatric Infectious Diseases, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan)

  • Hirokazu Seto

    (Department of Chemical Engineering, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan)

  • Takashi Kondo

    (Murata Manufacturing Co., Ltd., 1-10-1 Higashikotari, Nagaokakyo 617-8555, Japan)

  • Yoshio Ichinose

    (Kenya Research Station, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan)

  • Makoto Hasegawa

    (Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama 526-0829, Japan)

Abstract

Metal mesh devices (MMDs) are novel materials that enable the precise separation of particles by size. Structurally, MMDs consist of a periodic arrangement of square apertures of characteristic shapes and sizes on a thin nickel membrane. The present study describes the separation of aerosol particles using palm-top-size collection devices equipped with three types of MMDs differing in pore size. Aerosols were collected at a farm located in the suburbs of Nairobi, Kenya; aerosol particles were isolated, and pathogenic bacteria were identified in this microflora by next-generation sequencing analysis. The composition of the microflora in aerosol particles was found to depend on particle size. Gene fragments were obtained from the collected aerosols by PCR using primers specific for the genus Mycobacterium . This analysis showed that Mycobacterium obuense , a non-tuberculous species of mycobacteria that causes lung diseases, was present in these aerosols. These findings showed that application of this MMD analytical protocol to aerosol particles can facilitate the investigation of airborne pathogenic bacteria.

Suggested Citation

  • Xiaobo Yin & Seiji Kamba & Koki Yamamoto & Atsushi Ogura & Ernest Apondi Wandera & Mohammad Monir Shah & Hirokazu Seto & Takashi Kondo & Yoshio Ichinose & Makoto Hasegawa, 2022. "Analysis of Environmental and Pathogenic Bacteria Attached to Aerosol Particles Size-Separated with a Metal Mesh Device," IJERPH, MDPI, vol. 19(9), pages 1-13, May.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:9:p:5773-:d:811718
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/19/9/5773/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/19/9/5773/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Les Dethlefsen & Sue Huse & Mitchell L Sogin & David A Relman, 2008. "The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA Sequencing," PLOS Biology, Public Library of Science, vol. 6(11), pages 1-18, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Patrick D Schloss, 2009. "A High-Throughput DNA Sequence Aligner for Microbial Ecology Studies," PLOS ONE, Public Library of Science, vol. 4(12), pages 1-9, December.
    2. Chang-Ro Lee & Ill Hwan Cho & Byeong Chul Jeong & Sang Hee Lee, 2013. "Strategies to Minimize Antibiotic Resistance," IJERPH, MDPI, vol. 10(9), pages 1-32, September.
    3. Iyiola Olatunji Oladunjoye & Yusuf Amuda Tajudeen & Habeebullah Jayeola Oladipo & Mona Said El-Sherbini, 2022. "Planetary Health and Traditional Medicine: A Potential Synergistic Approach to Tackle Antimicrobial Resistance," Challenges, MDPI, vol. 13(1), pages 1-10, June.
    4. Oliver Aasmets & Kertu Liis Krigul & Kreete Lüll & Andres Metspalu & Elin Org, 2022. "Gut metagenome associations with extensive digital health data in a volunteer-based Estonian microbiome cohort," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    5. Eman M Fouda, 2017. "Airway Microbiota and Allergic Diseases: Clinical Implications," International Journal of Pulmonary & Respiratory Sciences, Juniper Publishers Inc., vol. 1(5), pages 1-5, May.
    6. Lena Takayasu & Wataru Suda & Eiichiro Watanabe & Shinji Fukuda & Kageyasu Takanashi & Hiroshi Ohno & Misako Takayasu & Hideki Takayasu & Masahira Hattori, 2017. "A 3-dimensional mathematical model of microbial proliferation that generates the characteristic cumulative relative abundance distributions in gut microbiomes," PLOS ONE, Public Library of Science, vol. 12(8), pages 1-20, August.
    7. Eman M Fouda, 2017. "Airway Microbiota and Allergic Diseases: Clinical Implications," International Journal of Pulmonary & Respiratory Sciences, Juniper Publishers Inc., vol. 1(5), pages 119-124, May.
    8. Bo-Young Hong & Michel V Furtado Araujo & Linda D Strausbaugh & Evimaria Terzi & Effie Ioannidou & Patricia I Diaz, 2015. "Microbiome Profiles in Periodontitis in Relation to Host and Disease Characteristics," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-14, May.
    9. Rebecca Flancman & Ameet Singh & J Scott Weese, 2018. "Evaluation of the impact of dental prophylaxis on the oral microbiota of dogs," PLOS ONE, Public Library of Science, vol. 13(6), pages 1-18, June.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:19:y:2022:i:9:p:5773-:d:811718. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.