IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v17y2020i4p1319-d322197.html
   My bibliography  Save this article

Investigation of the Interaction Mechanism of Perfluoroalkyl Carboxylic Acids with Human Serum Albumin by Spectroscopic Methods

Author

Listed:
  • Huilun Chen

    (School of Energy and Environmental Engineering, and Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China)

  • Qianyu Wang

    (School of Energy and Environmental Engineering, and Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China)

  • Yanping Cai

    (School of Energy and Environmental Engineering, and Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China)

  • Rongfang Yuan

    (School of Energy and Environmental Engineering, and Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China)

  • Fei Wang

    (School of Energy and Environmental Engineering, and Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China)

  • Beihai Zhou

    (School of Energy and Environmental Engineering, and Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China)

Abstract

Perfluoroalkyl carboxylic acids (PFCAs) are some of the most significant pollutants in human serum, and are reported to be potentially toxic to humans. In this study, the binding mechanism of PFCAs with different carbon lengths to human serum albumin (HSA) was studied at the molecular level by means of fluorescence spectroscopy under simulated physiological conditions and molecular modeling. Fluorescence data indicate that PFCAs with a longer carbon chain have a stronger fluorescence quenching ability. Perfluorobutanoic acid (PFBA) and perfluorohexanoic acid (PFHxA) had little effect on HSA. Fluorescence quenching of HSA by perfluorooctanoic acid (PFOA) and perfluorodecanoic acid (PFDA) was a static process that formed a PFCA–HSA complex. Electrostatic interactions were the main intermolecular forces between PFOA and HSA, while hydrogen bonding and van der Waals interactions played important roles in the combination of PFDA and HSA. In fact, the binding of PFDA to HSA was stronger than that of PFOA as supported by fluorescence quenching and molecular docking. In addition, infrared spectroscopy demonstrated that the binding of PFOA/PFDA resulted in a sharp decrease in the β-sheet and α-helix conformations of HSA. Our results indicated that the carbon chain length of PFCAs had a great impact on its binding affinity, and that PFCAs with longer carbon chains bound more strongly.

Suggested Citation

  • Huilun Chen & Qianyu Wang & Yanping Cai & Rongfang Yuan & Fei Wang & Beihai Zhou, 2020. "Investigation of the Interaction Mechanism of Perfluoroalkyl Carboxylic Acids with Human Serum Albumin by Spectroscopic Methods," IJERPH, MDPI, vol. 17(4), pages 1-12, February.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:4:p:1319-:d:322197
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/17/4/1319/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/17/4/1319/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Kristen M. Rappazzo & Evan Coffman & Erin P. Hines, 2017. "Exposure to Perfluorinated Alkyl Substances and Health Outcomes in Children: A Systematic Review of the Epidemiologic Literature," IJERPH, MDPI, vol. 14(7), pages 1-22, June.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yanping Cai & Haiyan Chen & Huilun Chen & Haiqing Li & Shuo Yang & Fei Wang, 2019. "Evaluation of Single and Joint Toxicity of Perfluorinated Carboxylic Acids and Copper to Metal-Resistant Arthrobacter Strains," IJERPH, MDPI, vol. 16(1), pages 1-11, January.
    2. Tiina Mattila & Tiina Santonen & Helle Raun Andersen & Andromachi Katsonouri & Tamás Szigeti & Maria Uhl & Wojciech Wąsowicz & Rosa Lange & Beatrice Bocca & Flavia Ruggieri & Marike Kolossa-Gehring & , 2021. "Scoping Review—The Association between Asthma and Environmental Chemicals," IJERPH, MDPI, vol. 18(3), pages 1-13, February.
    3. Marialuisa Menegatto & Sara Lezzi & Michele Musolino & Adriano Zamperini, 2022. "The Psychological Impact of Per- and Poly-Fluoroalkyl Substances (PFAS) Pollution in the Veneto Region, Italy: A Qualitative Study with Parents," IJERPH, MDPI, vol. 19(22), pages 1-22, November.
    4. Blake Langenbach & Mark Wilson, 2021. "Per- and Polyfluoroalkyl Substances (PFAS): Significance and Considerations within the Regulatory Framework of the USA," IJERPH, MDPI, vol. 18(21), pages 1-17, October.
    5. Hussein F. Hassan & Haneen Bou Ghanem & Joelle Abi Kharma & Mohamad G. Abiad & Jomana Elaridi & Maya Bassil, 2023. "Perfluorooctanoic Acid and Perfluorooctane Sulfonate in Human Milk: First Survey from Lebanon," IJERPH, MDPI, vol. 20(1), pages 1-14, January.
    6. Elsi Haverinen & Mariana F. Fernandez & Vicente Mustieles & Hanna Tolonen, 2021. "Metabolic Syndrome and Endocrine Disrupting Chemicals: An Overview of Exposure and Health Effects," IJERPH, MDPI, vol. 18(24), pages 1-19, December.
    7. Frances M. Nilsen & Jazmin D.C. Ruiz & Nicolle S. Tulve, 2020. "A Meta-Analysis of Stressors from the Total Environment Associated with Children’s General Cognitive Ability," IJERPH, MDPI, vol. 17(15), pages 1-35, July.
    8. Charlotte Stübner & Christel Nielsen & Kristina Jakobsson & Christopher Gillberg & Carmela Miniscalco, 2023. "Early-Life Exposure to Perfluoroalkyl Substances (PFAS) and Child Language and Communication Development: A Systematic Review," IJERPH, MDPI, vol. 20(24), pages 1-20, December.
    9. Siti Suhana Abdullah Soheimi & Amirah Abdul Rahman & Normala Abd Latip & Effendi Ibrahim & Siti Hamimah Sheikh Abdul Kadir, 2021. "Understanding the Impact of Perfluorinated Compounds on Cardiovascular Diseases and Their Risk Factors: A Meta-Analysis Study," IJERPH, MDPI, vol. 18(16), pages 1-22, August.
    10. Nicole Marie Brennan & Abigail Teresa Evans & Meredith Kate Fritz & Stephanie Allison Peak & Haley Elizabeth von Holst, 2021. "Trends in the Regulation of Per- and Polyfluoroalkyl Substances (PFAS): A Scoping Review," IJERPH, MDPI, vol. 18(20), pages 1-28, October.
    11. Laura Franza & Rossella Cianci, 2021. "Pollution, Inflammation, and Vaccines: A Complex Crosstalk," IJERPH, MDPI, vol. 18(12), pages 1-16, June.
    12. Qi Meng & Kosuke Inoue & Beate Ritz & Jørn Olsen & Zeyan Liew, 2018. "Prenatal Exposure to Perfluoroalkyl Substances and Birth Outcomes; An Updated Analysis from the Danish National Birth Cohort," IJERPH, MDPI, vol. 15(9), pages 1-15, August.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:17:y:2020:i:4:p:1319-:d:322197. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.