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The Association between Splenocyte Apoptosis and Alterations of Bax, Bcl-2 and Caspase-3 mRNA Expression, and Oxidative Stress Induced by Dietary Nickel Chloride in Broilers

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  • Jianying Huang

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Hengmin Cui

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Xi Peng

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Jing Fang

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Zhicai Zuo

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Junliang Deng

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Bangyuan Wu

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

Abstract

Two hundred and forty avian broilers were equally divided into four groups, and raised with a corn-soybean basal diet or the same diet supplemented with 300, 600, 900 mg/kg NiCl 2 for 42 days. Numbers or percentages of apoptotic splenocytes by flow cytometry (FCM) and TUNEL were higher ( p < 0.05 or p < 0.01) in the 300, 600 and 900 mg/kg groups than those in the control group. Results measured by qRT-PCR and ELISA showed that mRNA expression and contents were significantly higher ( p < 0.05 or p < 0.01) in Bax and Caspase-3, and were significantly lower ( p < 0.05 or p < 0.01) in Bcl-2 of the 300, 600 and 900 mg/kg groups. Also, the SOD, CAT and GSH-Px activities, and the ability to inhibit hydroxyl radical, and GSH contents were significantly decreased ( p < 0.05 or p < 0.01), and MDA contents were increased ( p < 0.05 or p < 0.01) in all groups. In conclusion, dietary NiCl 2 in excess of 300 mg/kg caused apoptosis, altered Bax, Bcl-2 and Caspase-3 mRNA expression levels and contents, and induced oxidative stress in the spleen. Also, splenocyte apoptosis was closely related to the alternations of Bax, Bcl-2 and Caspase-3 mRNA expression, and oxidative damage. The splenic immunity and blood filtration functions were impaired in broilers.

Suggested Citation

  • Jianying Huang & Hengmin Cui & Xi Peng & Jing Fang & Zhicai Zuo & Junliang Deng & Bangyuan Wu, 2013. "The Association between Splenocyte Apoptosis and Alterations of Bax, Bcl-2 and Caspase-3 mRNA Expression, and Oxidative Stress Induced by Dietary Nickel Chloride in Broilers," IJERPH, MDPI, vol. 10(12), pages 1-17, December.
    • Handle: RePEc:gam:jijerp:v:10:y:2013:i:12:p:7310-7326:d:31439
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    References listed on IDEAS

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    1. Bangyuan Wu & Hengmin Cui & Xi Peng & Jing Fang & Zhicai Zuo & Junliang Deng & Jianying Huang, 2013. "Dietary Nickel Chloride Induces Oxidative Intestinal Damage in Broilers," IJERPH, MDPI, vol. 10(6), pages 1-11, May.
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    Cited by:

    1. Xi Peng & Shengqiang Zhang & Jing Fang & Hengmin Cui & Zhicai Zuo & Junliang Deng, 2014. "Protective Roles of Sodium Selenite against Aflatoxin B 1 -Induced Apoptosis of Jejunum in Broilers," IJERPH, MDPI, vol. 11(12), pages 1-14, December.
    2. Ali Allam & Ahmed Abdeen & Hari Prasad Devkota & Samar S. Ibrahim & Gehan Youssef & Ahmed Soliman & Mohamed M. Abdel-Daim & Khalid J. Alzahrani & Khaled Shoghy & Samah F. Ibrahim & Mohamed Aboubakr, 2022. "N -Acetylcysteine Alleviated the Deltamethrin-Induced Oxidative Cascade and Apoptosis in Liver and Kidney Tissues," IJERPH, MDPI, vol. 19(2), pages 1-13, January.

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