IDEAS home Printed from https://ideas.repec.org/a/gam/jdataj/v5y2020i3p77-d407005.html
   My bibliography  Save this article

Dataset of Nile Red Fluorescence Readings with Different Yeast Strains, Solvents, and Incubation Times

Author

Listed:
  • Mauricio Ramirez-Castrillon

    (Research Group in Mycology (GIM/CICBA), Universidad Santiago de Cali, Calle 5 No. 62-00, Santiago de Cali, Colombia
    Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500 Prédios 43421/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil)

  • Victoria P. Jaramillo-Garcia

    (Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500 Prédios 43421/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil)

  • Helio Lopes Barros

    (New Organic Materials and Forensic Chemistry Laboratory (LNMO-QF), Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil)

  • João A. Pêgas Henriques

    (Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500 Prédios 43421/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil)

  • Valter Stefani

    (New Organic Materials and Forensic Chemistry Laboratory (LNMO-QF), Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil
    In Memoriam.)

  • Patricia Valente

    (Department of Microbiology, Immunology and Parasitology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS CEP 90050-170, Brazil)

Abstract

We used Nile red to estimate lipid content in oleaginous yeasts using a high-throughput approach. We measured the fluorescence intensity of Nile red using different solvents, yeast strains, and incubation times in optimized excitation/emission wavelengths. The data show the relative fluorescence units (RFU) for Nile red excitation, using 1× PBS, 1× PBS and 5% v / v isopropyl alcohol, 50% v / v glycerol, culture medium A-gly broth, and A-gly broth supplemented with 5% v / v DMSO. In addition, we showed the RFU for the Nile red dye for different oleaginous and non-oleaginous yeast strains, such as Meyerozyma guilliermondii BI281A, Yarrowia lipolytica QU21 and Saccharomyces cerevisiae MRC164. Other measurements of lipid accumulation kinetics were shown for the above and additional yeast strains. These datasets provide the guidelines to obtain the optimal solvent system and the minimal interaction time for the Nile red dye to enter in the cells and obtain a stable readout.

Suggested Citation

  • Mauricio Ramirez-Castrillon & Victoria P. Jaramillo-Garcia & Helio Lopes Barros & João A. Pêgas Henriques & Valter Stefani & Patricia Valente, 2020. "Dataset of Nile Red Fluorescence Readings with Different Yeast Strains, Solvents, and Incubation Times," Data, MDPI, vol. 5(3), pages 1-6, September.
    • Handle: RePEc:gam:jdataj:v:5:y:2020:i:3:p:77-:d:407005
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2306-5729/5/3/77/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2306-5729/5/3/77/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Patel, Alok & Pruthi, Vikas & Pruthi, Parul A., 2019. "Innovative screening approach for the identification of triacylglycerol accumulating oleaginous strains," Renewable Energy, Elsevier, vol. 135(C), pages 936-944.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Alok Patel & Liwen Mu & Yijun Shi & Ulrika Rova & Paul Christakopoulos & Leonidas Matsakas, 2021. "Single-Cell Oils from Oleaginous Microorganisms as Green Bio-Lubricants: Studies on Their Tribological Performance," Energies, MDPI, vol. 14(20), pages 1-17, October.

    More about this item

    Keywords

    ;
    ;
    ;
    ;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jdataj:v:5:y:2020:i:3:p:77-:d:407005. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.