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Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops

Author

Listed:
  • Joel P. Hague

    () (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

  • Stephen L. Dellaporta

    () (Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA)

  • Maria A. Moreno

    () (Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA)

  • Chip Longo

    () (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

  • Kimberly Nelson

    () (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

  • Albert P. Kausch

    () (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

Abstract

Advanced genetic and biotechnology tools will be required to realize the full potential of food and bioenergy crops. Given current regulatory concerns, many transgenic traits might never be deregulated for commercial release without a robust gene confinement strategy in place. The potential for transgene flow from genetically modified (GM) crops is widely known. Pollen-mediated transfer is a major component of gene flow in flowering plants and therefore a potential avenue for the escape of transgenes from GM crops. One approach for preventing and/or mitigating transgene flow is the production of trait linked pollen sterility. To evaluate the feasibility of generating pollen sterility lines for gene confinement and breeding purposes we tested the utility of a promoter ( Zm13 Pro) from a maize pollen-specific gene ( Zm13 ) for driving expression of the reporter gene GUS and the cytotoxic gene barnase in transgenic rice ( Oryza sativa ssp. Japonica cv. Nipponbare) as a monocot proxy for bioenergy grasses. This study demonstrates that the Zm13 promoter can drive pollen-specific expression in stably transformed rice and may be useful for gametophytic transgene confinement and breeding strategies by pollen sterility in food and bioenergy crops.

Suggested Citation

  • Joel P. Hague & Stephen L. Dellaporta & Maria A. Moreno & Chip Longo & Kimberly Nelson & Albert P. Kausch, 2012. "Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops," Agriculture, MDPI, Open Access Journal, vol. 2(4), pages 1-21, October.
  • Handle: RePEc:gam:jagris:v:2:y:2012:i:4:p:295-315:d:20685
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    References listed on IDEAS

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    1. anonymous, 2000. "The brave new world of agriculture," EconSouth, Federal Reserve Bank of Atlanta, issue Q3, pages 8-13.
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    More about this item

    Keywords

    bioenergy; gene confinement; GM crops; transgenic plants; pollen sterility; regulatory concerns; agricultural regulation; environmental regulation; gametophyte; Oryza sativa;

    JEL classification:

    • Q1 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture
    • Q10 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - General
    • Q11 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - Aggregate Supply and Demand Analysis; Prices
    • Q12 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - Micro Analysis of Farm Firms, Farm Households, and Farm Input Markets
    • Q13 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - Agricultural Markets and Marketing; Cooperatives; Agribusiness
    • Q14 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - Agricultural Finance
    • Q15 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - Land Ownership and Tenure; Land Reform; Land Use; Irrigation; Agriculture and Environment
    • Q16 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - R&D; Agricultural Technology; Biofuels; Agricultural Extension Services
    • Q17 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - Agriculture in International Trade
    • Q18 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Agriculture - - - Agricultural Policy; Food Policy

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