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Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops

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  • Joel P. Hague

    (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

  • Stephen L. Dellaporta

    (Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA)

  • Maria A. Moreno

    (Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA)

  • Chip Longo

    (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

  • Kimberly Nelson

    (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

  • Albert P. Kausch

    (Department of Cell and Molecular Biology, University of Rhode Island, West Kingston, RI 02892, USA)

Abstract

Advanced genetic and biotechnology tools will be required to realize the full potential of food and bioenergy crops. Given current regulatory concerns, many transgenic traits might never be deregulated for commercial release without a robust gene confinement strategy in place. The potential for transgene flow from genetically modified (GM) crops is widely known. Pollen-mediated transfer is a major component of gene flow in flowering plants and therefore a potential avenue for the escape of transgenes from GM crops. One approach for preventing and/or mitigating transgene flow is the production of trait linked pollen sterility. To evaluate the feasibility of generating pollen sterility lines for gene confinement and breeding purposes we tested the utility of a promoter ( Zm13 Pro) from a maize pollen-specific gene ( Zm13 ) for driving expression of the reporter gene GUS and the cytotoxic gene barnase in transgenic rice ( Oryza sativa ssp. Japonica cv. Nipponbare) as a monocot proxy for bioenergy grasses. This study demonstrates that the Zm13 promoter can drive pollen-specific expression in stably transformed rice and may be useful for gametophytic transgene confinement and breeding strategies by pollen sterility in food and bioenergy crops.

Suggested Citation

  • Joel P. Hague & Stephen L. Dellaporta & Maria A. Moreno & Chip Longo & Kimberly Nelson & Albert P. Kausch, 2012. "Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops," Agriculture, MDPI, vol. 2(4), pages 1-21, October.
    • Handle: RePEc:gam:jagris:v:2:y:2012:i:4:p:295-315:d:20685
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    References listed on IDEAS

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    1. Vipula K. Shukla & Yannick Doyon & Jeffrey C. Miller & Russell C. DeKelver & Erica A. Moehle & Sarah E. Worden & Jon C. Mitchell & Nicole L. Arnold & Sunita Gopalan & Xiangdong Meng & Vivian M. Choi &, 2009. "Precise genome modification in the crop species Zea mays using zinc-finger nucleases," Nature, Nature, vol. 459(7245), pages 437-441, May.
    2. Jeffrey A. Townsend & David A. Wright & Ronnie J. Winfrey & Fengli Fu & Morgan L. Maeder & J. Keith Joung & Daniel F. Voytas, 2009. "High-frequency modification of plant genes using engineered zinc-finger nucleases," Nature, Nature, vol. 459(7245), pages 442-445, May.
    3. Matthew H. Porteus, 2009. "Zinc fingers on target," Nature, Nature, vol. 459(7245), pages 337-338, May.
    4. Muhammad Sarwar Khan, 2005. "Engineered male sterility," Nature, Nature, vol. 436(7052), pages 783-785, August.
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