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A Preliminary Investigation into Heavy Metal Tolerance in Pseudomonas Isolates: Does the Isolation Site Have an Effect?

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  • Alessandro De Santis

    (Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Via Napoli 25, 71122 Foggia, Italy)

  • Antonio Bevilacqua

    (Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Via Napoli 25, 71122 Foggia, Italy)

  • Angela Racioppo

    (Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Via Napoli 25, 71122 Foggia, Italy)

  • Barbara Speranza

    (Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Via Napoli 25, 71122 Foggia, Italy)

  • Maria Rosaria Corbo

    (Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Via Napoli 25, 71122 Foggia, Italy)

  • Clelia Altieri

    (Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Via Napoli 25, 71122 Foggia, Italy)

  • Milena Sinigaglia

    (Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Via Napoli 25, 71122 Foggia, Italy)

Abstract

One hundred presumptive Pseudomonas isolates, recovered from 15 sites impacted by anthropogenic activity in the Foggia district (Italy), were screened for key adaptive and functional traits important for environmental applications. The isolates were phenotypically characterized for their ability to grow under combined pH (5.0–8.0) and temperature (15–37 °C) conditions, to produce proteolytic enzymes, pigments, and exopolysaccharides, and to tolerate SDS. Moreover, the resistance to six environmentally relevant heavy metals (Cd, Co, Cu, Ni, Zn, As) was qualitatively assessed. The results highlighted wide inter-strain variability, with distinct clusters of isolates showing unique combinations of stress tolerance, enzymatic potential, and resistance profile. PERMANOVA analysis revealed significant effects of both the isolation site and the metal type, as well as their interaction, on the observed resistance patterns. A subset of isolates showed co-tolerance to elevated temperatures and heavy metals. These findings offer an initial yet insightful overview of the adaptive diversity of soil-derived Pseudomonas , laying the groundwork for the rational selection of strains for bioaugmentation in contaminated soils.

Suggested Citation

  • Alessandro De Santis & Antonio Bevilacqua & Angela Racioppo & Barbara Speranza & Maria Rosaria Corbo & Clelia Altieri & Milena Sinigaglia, 2025. "A Preliminary Investigation into Heavy Metal Tolerance in Pseudomonas Isolates: Does the Isolation Site Have an Effect?," Agriculture, MDPI, vol. 15(15), pages 1-17, August.
    • Handle: RePEc:gam:jagris:v:15:y:2025:i:15:p:1692-:d:1718136
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    References listed on IDEAS

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    1. Yingjin Song & Ruiyi Li & Guanyi Chen & Beibei Yan & Lei Zhong & Yuxin Wang & Yihang Li & Jinlei Li & Yingxiu Zhang, 2021. "Bibliometric Analysis of Current Status on Bioremediation of Petroleum Contaminated Soils during 2000–2019," IJERPH, MDPI, vol. 18(16), pages 1-20, August.
    2. Gaowen Yang & Masahiro Ryo & Julien Roy & Daniel R. Lammel & Max-Bernhard Ballhausen & Xin Jing & Xuefeng Zhu & Matthias C. Rillig, 2022. "Multiple anthropogenic pressures eliminate the effects of soil microbial diversity on ecosystem functions in experimental microcosms," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    3. Gayathiri Verasoundarapandian & Chiew-Yen Wong & Noor Azmi Shaharuddin & Claudio Gomez-Fuentes & Azham Zulkharnain & Siti Aqlima Ahmad, 2021. "A Review and Bibliometric Analysis on Applications of Microbial Degradation of Hydrocarbon Contaminants in Arctic Marine Environment at Metagenomic and Enzymatic Levels," IJERPH, MDPI, vol. 18(4), pages 1-30, February.
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