IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v14y2024i9p1545-d1473038.html
   My bibliography  Save this article

Combined Transcriptome and Metabolome Analysis of Alfalfa Responses to Aphid Infestation

Author

Listed:
  • Hao Liu

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China
    These authors contributed equally to this work.)

  • Ming Xu

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China
    These authors contributed equally to this work.)

  • Yuhan Guo

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Zhencuo Dan

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Xin Liu

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Jiayi Zhang

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Cong Li

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Shizhen Jia

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Lei Jia

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Ailing Yu

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

  • Lili Cong

    (College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China
    Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao 266109, China)

Abstract

Alfalfa ( Medicago sativa L.) is an economically important forage legume. As a result of the extensive and intensive cultivation of alfalfa planting and the gradual expansion of planting areas, pest occurrence has increased in frequency. Aphids are one of the main pests that threaten the growth and productivity of alfalfa. After an aphid outbreak, alfalfa yield and quality are commonly greatly reduced. At present, there are few studies on alfalfa plants infested with aphids, so it is imperative to study the regulatory mechanisms of aphid infestation tolerance in alfalfa. In this study, alfalfa plants from the variety “Wudi” were investigated, and pea aphids were selected for inoculation. The transcriptome and metabolome data were analyzed at three time points (0 d, 1 d, and 4 d), revealing 3458 differentially expressed genes and 358 differential metabolites. Trend analysis of DEGs and DAMs revealed that the former were significantly enriched in three distinct trends, whereas the latter were significantly enriched in only one. Moreover, 117 important hub genes associated with alfalfa response to aphid infestation were screened by WGCNA. By mapping DEGs and DAMs to KEGG pathways, it was found that the “phenylpropanoid biosynthesis”, “flavonoid biosynthesis”, and “isoflavonoid biosynthesis” pathways play an important role in alfalfa responses to aphid infestation. These results further elucidate the regulatory mechanism of alfalfa in response to aphid infestation and provide valuable information for breeding new aphid-resistant plant varieties.

Suggested Citation

  • Hao Liu & Ming Xu & Yuhan Guo & Zhencuo Dan & Xin Liu & Jiayi Zhang & Cong Li & Shizhen Jia & Lei Jia & Ailing Yu & Lili Cong, 2024. "Combined Transcriptome and Metabolome Analysis of Alfalfa Responses to Aphid Infestation," Agriculture, MDPI, vol. 14(9), pages 1-17, September.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:9:p:1545-:d:1473038
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/14/9/1545/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/14/9/1545/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Lijiao Gu & Pengyun Chen & Shuxun Yu, 2023. "The cytochrome P450 gene GhCYP94C1 is involved in drought stress in upland cotton (Gossypium hirsutum L.)," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 59(4), pages 189-195.
    2. Yajun Ma & Guozhu Yang & Ruijun Duan & Xiaoan Li & Siheng Zeng & Yajun Yan & Ce Zheng & Yueming Hu, 2024. "Transcriptome analysis of alfalfa (Medicago sativa L.) roots reveals overwintering changes in different varieties," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 60(2), pages 97-104.
    3. Haitao Chen & Yan Zeng & Yongzhi Yang & Lingli Huang & Bolin Tang & He Zhang & Fei Hao & Wei Liu & Youhan Li & Yanbin Liu & Xiaoshuang Zhang & Ru Zhang & Yesheng Zhang & Yongxin Li & Kun Wang & Hua He, 2020. "Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Lixin Zhang & Yuanbo Jiang & Minhua Yin & Yi Ling & Haiyan Li & Yuanxiang Gan & Changfa Yue & Yanlin Ma & Yanxia Kang & Guangping Qi & Yayu Wang, 2025. "Optimizing Phosphorus Fertilization Management Is Conducive to Improving Alfalfa Yield and Quality: A Meta-Analysis," Agriculture, MDPI, vol. 15(8), pages 1-22, April.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Botong Zhou & Ping Hu & Guichun Liu & Zhou Chang & Zhiwei Dong & Zihe Li & Yuan Yin & Zunzhe Tian & Ge Han & Wen Wang & Xueyan Li, 2024. "Evolutionary patterns and functional effects of 3D chromatin structures in butterflies with extensive genome rearrangements," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Aiping Song & Jiangshuo Su & Haibin Wang & Zhongren Zhang & Xingtan Zhang & Yves Peer & Fei Chen & Weimin Fang & Zhiyong Guan & Fei Zhang & Zhenxing Wang & Likai Wang & Baoqing Ding & Shuang Zhao & Li, 2023. "Analyses of a chromosome-scale genome assembly reveal the origin and evolution of cultivated chrysanthemum," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Yanting Shen & Wanying Li & Ying Zeng & Zhipeng Li & Yiqiong Chen & Jixiang Zhang & Hong Zhao & Lingfang Feng & Dongming Ma & Xiaolu Mo & Puyue Ouyang & Lili Huang & Zheng Wang & Yuannian Jiao & Hong-, 2022. "Chromosome-level and haplotype-resolved genome provides insight into the tetraploid hybrid origin of patchouli," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:14:y:2024:i:9:p:1545-:d:1473038. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.