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Whole-Plant Measure of Temperature-Induced Changes in the Cytosolic pH of Potato Plants Using Genetically Encoded Fluorescent Sensor Pt-GFP

Author

Listed:
  • Anna Pecherina

    (Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

  • Marina Grinberg

    (Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

  • Maria Ageyeva

    (Department of Biochemistry and Biotechnology, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

  • Tatiana Zdobnova

    (Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

  • Maria Ladeynova

    (Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

  • Andrey Yudintsev

    (Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

  • Vladimir Vodeneev

    (Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

  • Anna Brilkina

    (Department of Biochemistry and Biotechnology, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia)

Abstract

Cytosolic pH (pH cyt ) regulates a wide range of cellular processes in plants. Changes in pH cyt occurring under the effect of different stressors can participate in signal transmission. The dynamics of pH cyt under the action of external factors, including significant factors for open ground crops such as temperature, remains poorly understood, which is largely due to the difficulty of intracellular pH registration using standard methods. In this work, model plants of potato (one of the essential crops) expressing a fluorescent ratiometric pH sensor Pt-GFP were created. The calibration obtained in vivo allowed for the determination of the pH cyt values of the cells of the leaves, which is 7.03 ± 0.03 pH. Cooling of the whole leaf caused depolarization and rapid acidification of the cytosol, the amplitude of which depended on the cooling strength, amounting to about 0.2 pH units when cooled by 15 °C. When the temperature rises to 35–40 °C, the cytosol was alkalized by 0.2 pH units. Heating above the threshold temperature caused the acidification of cytosol and generation of variation potential. The observed rapid changes in pH cyt can be associated with changes in the activity of H + -ATPases, which was confirmed by inhibitory analysis.

Suggested Citation

  • Anna Pecherina & Marina Grinberg & Maria Ageyeva & Tatiana Zdobnova & Maria Ladeynova & Andrey Yudintsev & Vladimir Vodeneev & Anna Brilkina, 2021. "Whole-Plant Measure of Temperature-Induced Changes in the Cytosolic pH of Potato Plants Using Genetically Encoded Fluorescent Sensor Pt-GFP," Agriculture, MDPI, vol. 11(11), pages 1-19, November.
    • Handle: RePEc:gam:jagris:v:11:y:2021:i:11:p:1131-:d:676959
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    References listed on IDEAS

    as
    1. Olga Escuredo & Ana Seijo-Rodríguez & M. Shantal Rodríguez-Flores & Laura Meno & M. Carmen Seijo, 2020. "Changes in the Morphological Characteristics of Potato Plants Attributed to Seasonal Variability," Agriculture, MDPI, vol. 10(4), pages 1-14, March.
    2. Vashisht, B.B. & Nigon, T. & Mulla, D.J. & Rosen, C. & Xu, H. & Twine, T. & Jalota, S.K., 2015. "Adaptation of water and nitrogen management to future climates for sustaining potato yield in Minnesota: Field and simulation study," Agricultural Water Management, Elsevier, vol. 152(C), pages 198-206.
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