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Continuous recycling of enzymes during production of lignocellulosic bioethanol in demonstration scale

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  • Haven, Mai Østergaard
  • Lindedam, Jane
  • Jeppesen, Martin Dan
  • Elleskov, Michael
  • Rodrigues, Ana Cristina
  • Gama, Miguel
  • Jørgensen, Henning
  • Felby, Claus

Abstract

Recycling of enzymes in production of lignocellulosic bioethanol has been tried for more than 30years. So far, the successes have been few and the experiments have been carried out at conditions far from those in an industrially feasible process. Here we have tested continuous enzyme recycling at demonstration scale using industrial process conditions (high dry matter content and low enzyme dosage) for a period of eight days. The experiment was performed at the Inbicon demonstration plant (Kalundborg, Denmark) capable of converting four tonnes of wheat straw per hour. 20% of the fermentation broth was recycled to the hydrolysis reactor while enzyme dosage was reduced by 5%. The results demonstrate that recycling enzymes by this method can reduce overall enzyme consumption and may also increase the ethanol concentrations in the fermentation broth. Our results further show that recycling fermentation broth also opens up the possibility of lowering the dry matter content in hydrolysis and fermentation while still maintaining high ethanol concentrations.

Suggested Citation

  • Haven, Mai Østergaard & Lindedam, Jane & Jeppesen, Martin Dan & Elleskov, Michael & Rodrigues, Ana Cristina & Gama, Miguel & Jørgensen, Henning & Felby, Claus, 2015. "Continuous recycling of enzymes during production of lignocellulosic bioethanol in demonstration scale," Applied Energy, Elsevier, vol. 159(C), pages 188-195.
  • Handle: RePEc:eee:appene:v:159:y:2015:i:c:p:188-195
    DOI: 10.1016/j.apenergy.2015.08.062
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    Cited by:

    1. Chen, Jiaxin & Zhang, Biying & Luo, Lingli & Zhang, Fan & Yi, Yanglei & Shan, Yuanyuan & Liu, Bianfang & Zhou, Yuan & Wang, Xin & Lü, Xin, 2021. "A review on recycling techniques for bioethanol production from lignocellulosic biomass," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    2. Mesa, Leyanis & Martínez, Yenisleidy & Barrio, Edenny & González, Erenio, 2017. "Desirability function for optimization of Dilute Acid pretreatment of sugarcane straw for ethanol production and preliminary economic analysis based in three fermentation configurations," Applied Energy, Elsevier, vol. 198(C), pages 299-311.
    3. Byun, Jaewon & Han, Jeehoon, 2016. "Process synthesis and analysis for catalytic conversion of lignocellulosic biomass to fuels: Separate conversion of cellulose and hemicellulose using 2-sec-butylphenol (SBP) solvent," Applied Energy, Elsevier, vol. 171(C), pages 483-490.

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