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Association between conventional semen variables and sperm freezability in rams

Author

Listed:
  • Aizhan Makhanbetova

    (Meat Sheep Breeding Department, Kazakh Research Institute of Livestock and Fodder Production, Almaty, Republic of Kazakhstan)

  • Filipp Georgijevič Savvulidi

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sceinces (CULS) Prague, Prague, Czech Republic)

  • Martin Ptáček

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sceinces (CULS) Prague, Prague, Czech Republic)

  • Lucie Langerová

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sceinces (CULS) Prague, Prague, Czech Republic)

  • Beybit Kulataev

    (Meat Sheep Breeding Department, Kazakh Research Institute of Livestock and Fodder Production, Almaty, Republic of Kazakhstan)

  • Nurlan Malmakov

    (Meat Sheep Breeding Department, Kazakh Research Institute of Livestock and Fodder Production, Almaty, Republic of Kazakhstan)

Abstract

Assessments of routine ejaculated samples were used to evaluate the conventional semen variables (sperm motility, semen volume, sperm concentration) as initial decision-making criteria to freeze or not to freeze. However, the association of these attributes to the cryotolerance of ram sperm has not been adequately studied yet. The aim of this study was therefore to analyse if the semen volume, sperm motility and concentration can be used to predict post-thaw ram sperm motility. Additionally, in the present study we tested if the order of ejaculate sampling (first versus second, within a single collection session) and the breed of rams influenced cryopreservation outcomes.

Suggested Citation

  • Aizhan Makhanbetova & Filipp Georgijevič Savvulidi & Martin Ptáček & Lucie Langerová & Beybit Kulataev & Nurlan Malmakov, 2025. "Association between conventional semen variables and sperm freezability in rams," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 70(3), pages 93-101.
  • Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:3:id:185-2024-cjas
    DOI: 10.17221/185/2024-CJAS
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    References listed on IDEAS

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    1. Anežka Málková & Martin Ptáček & Filipp Georgijevič Savvulidi & Szabolcs Tamás Nagy & Luděk Stádník, 2024. "Effects of age and litter-of-origin on cryopreserved spermatozoa in Sumava rams," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 69(4), pages 129-138.
    2. P. Padrik & T. Hallap & T. Kaart & T. Bulitko & Ü. Jaakma, 2012. "Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(10), pages 490-497.
    3. Filipp Georgijevic Savvulidi & Martin Ptacek & Anezka Malkova & Jakub Beranek & Ludek Stadnik, 2021. "Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 66(2), pages 55-64.
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