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Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023

Author

Listed:
  • Filipp Georgijevič Savvulidi

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

  • Martin Ptáček

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

  • Szabolcs Nagy

    (Institute of Animal Sciences, Hungarian University of Agriculture and Life Sciences, Georgikon Campus, Keszthely, Hungary)

  • Luděk Stádník

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

Abstract

Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality.

Suggested Citation

  • Filipp Georgijevič Savvulidi & Martin Ptáček & Szabolcs Nagy & Luděk Stádník, 2023. "Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 68(12), pages 469-485.
    • Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:12:id:152-2023-cjas
    DOI: 10.17221/152/2023-CJAS
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    References listed on IDEAS

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    1. Filipp Georgijevic Savvulidi & Martin Ptacek & Anezka Malkova & Jakub Beranek & Ludek Stadnik, 2021. "Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 66(2), pages 55-64.
    2. Jan Pytlík & Filipp Georgijevič Savvulidi & Jaromír Ducháček & Radim Codl & Marek Vrhel & Szabolcs Nagy & Luděk Stádník, 2022. "Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 67(3), pages 75-86.
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