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Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program

Author

Listed:
  • Filipp Georgijevic Savvulidi
  • Martin Ptacek

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

  • Anezka Malkova

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

  • Jakub Beranek

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

  • Ludek Stadnik

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

Abstract

The aim of the present study was to optimize the conventional method of sperm freezing in liquid nitrogen (LN2) vapour for successful cryopreservation of Wallachian ram sperm, the genetic resources of the Czech Republic. Sperm in straws were frozen using the conventional freezing method via a static exposure of sperm doses to LN2 vapour, or by four different modified freezing methods. Under modified freezing, straws were frozen by a discontinuous, time-dependent decremental change in the distance between the straws and the surface of LN2. The viability of sperm was evaluated by flow cytometry after sperm equilibration, and immediately after thawing. Besides the observed inter-sire and daily variation, the obtained results suggest the methodological weakness of the conventional freezing method via the static exposure of sperm doses to LN2 vapour. With the use of the optimized freezing procedure, all parameters of thawed sperm were significantly (P < 0.05) improved in comparison with the conventional method: percentage of thawed sperm viability increased up to 48.3%, percentage of sperm with plasma membrane damage after thawing decreased to 6.58%, percentage of sperm with acrosome damage decreased to 24.4%, and percentage of sperm with deteriorated mitochondrial activity decreased to 6.28%. In conclusion, our results suggest that an optimized freezing procedure should be routinely used instead of the conventional method to cryopreserve Wallachian ram sperm.

Suggested Citation

  • Filipp Georgijevic Savvulidi & Martin Ptacek & Anezka Malkova & Jakub Beranek & Ludek Stadnik, 2021. "Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 66(2), pages 55-64.
    • Handle: RePEc:caa:jnlcjs:v:66:y:2021:i:2:id:226-2020-cjas
    DOI: 10.17221/226/2020-CJAS
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    References listed on IDEAS

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    1. Marija Jovičić & Eva Chmelíková & Markéta Sedmíková, 2020. "Cryopreservation of boar semen," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 65(4), pages 115-123.
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    Cited by:

    1. Filipp Georgijevič Savvulidi & Martin Ptáček & Szabolcs Nagy & Luděk Stádník, 2023. "Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 68(12), pages 469-485.

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