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Synthesis and Evaluation of Chitosan Derivatives for Large Size DNA Delivery

Author

Listed:
  • Sankov V
  • Shagdarova B
  • Chudakov D
  • Reshetov P
  • Grechikhina M
  • Zubareva A
  • Varlamov V
  • Esipov R
  • Zubov V
  • Svirshchevskaya E

    (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia
    Lomonosov Moscow State University, Russia
    Institute of Bioengineering, Research Center of Biotechnology of the Russian, Academy of Sciences, Russia)

Abstract

Chitosan (Chi) is a natural inexpensive renewable biodegradable cationic polymer with promising potency for different applications including gene delivery. Multiple studies demonstrate that unmodified Chi shows poor cellular uptake leading to low transfection efficiency. Chi possesses multiple reactive groups used to obtain derivatives with modified properties. Numerous attempts in Chi modification demonstrated improved transfection efficacy. However a direct comparison of different Chi derivative was not done. This paper analyses the efficacy of large size DNA delivery by 24 Chi. samples. A рRFP pmKate2 reporter plasmid with MW 3065 kDa was used as a model DNA to transfect HEK293 cells. Among all only a set of hexanoyl-chitosan with molecular weight 20 kDa and substitution degree 10/15/30% was able to deliver the plasmid into cells at a significant level comparable with the efficacy of liposomal reagent Metafectene Pro.

Suggested Citation

  • Sankov V & Shagdarova B & Chudakov D & Reshetov P & Grechikhina M & Zubareva A & Varlamov V & Esipov R & Zubov V & Svirshchevskaya E, 2017. "Synthesis and Evaluation of Chitosan Derivatives for Large Size DNA Delivery," Organic & Medicinal Chemistry International Journal, Juniper Publishers Inc., vol. 1(4), pages 172-178, March.
    • Handle: RePEc:adp:jomcij:v:1:y:2017:i:4:p:172-178
    DOI: 10.19080/OMCIJ.2017.01.555572
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