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Isolation and Phenotypic Detection of Metallo- Beta-Lactamase (MBL)-Producing Klebsiella Species from Cow Anal Swabs

Author

Listed:
  • Ejikeugwu Chika
  • Oguejiofor Benigna
  • Ogene Lilian
  • Iroha Ifeanyichukwu

    (Department of Applied Microbiology, Ebonyi State University, Abakaliki)

  • Duru Carissa

    (Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, NIgeria)

  • Eluu Stanley

    (Department of Biological Sciences (Biotechnology Programme), Ebonyi State University, Abakaliki)

  • Ezeador Chika

    (Department of Microbiology and Parasitology, Nnamdi Azikiwe University Teaching Hospital (NAUTH), Nnewi, Nigeria)

Abstract

The production of β-lactamase enzymes such as metallo β-lactamases (MBLs) is one of the most distributed resistance mechanisms engineered by Gram-negative bacteria towards β-lactam antibiotics. MBLs confer on bacteria the ability to hydrolyze and become resistant to the carbapenems such as imipenem. This study was aimed at isolating and detecting the production of MBLs from Klebsiella species that emanated from anal swabs of cow in a local abattoir. A total of 31 anal swab samples were bacteriologically analyzed for the isolation of Klebsiella species which were identified using standard microbiological techniques. Antimicrobial susceptibility testing was carried out using the modified Kirby-Bauer disk diffusion method on Mueller-Hinton agar plates. The isolated Klebsiella species was screened for MBL production by determining their susceptibility to the carbapenems and this was confirmed using the EDTA-inhibition-based assay for phenotypic detection of MBLs. A total of 18 Klebsiella species was isolated from the cow anal swab samples

Suggested Citation

  • Ejikeugwu Chika & Oguejiofor Benigna & Ogene Lilian & Iroha Ifeanyichukwu & Duru Carissa & Eluu Stanley & Ezeador Chika, 2017. "Isolation and Phenotypic Detection of Metallo- Beta-Lactamase (MBL)-Producing Klebsiella Species from Cow Anal Swabs," Global Journal of Pharmacy & Pharmaceutical Sciences, Juniper Publishers Inc., vol. 2(3), pages 54-58, May.
    • Handle: RePEc:adp:jgjpps:v:2:y:2017:i:3:p:54-58
    DOI: 10.19080/GJPPS.2017.02.555586
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