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Evaluation of Optimal Doses for Gamma Rays and Hydrazine Hydrates in Lentil Genotypes

Author

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  • Rafiul Amin Laskar
  • Samiullah Khan

    (Mutation Breeding Lab., Department of Botany, India)

Abstract

Induced mutagenesis is an extremely powerful technique to create genetic variability that is either not exists in nature or not available to the breeders. The rate of induced mutation depends on the genotype and the degree of exposure. The present study documents the dose responses of potential physical (gamma rays) and chemical (hydrazine hydrates) mutagenic agents in the microsperma and macrosperma genotypes of lentil. The selection of optimal doses of mutagens through LD50 values, calculated based on the seed germination index, was carried out. The dose–response relationship between applied doses and the genotypes showed a linear increase in germination inhibition with increase in strength of mutagenic doses. The result of the present study showed the 0.4% in HZ, 400 Gy in gamma rays and 400 Gy+0.4% HZ in combination treatments were the maximum non-lethal strength of the respective mutagen for induction of mutation in the lentil genotypes DPL 62 and Pant L 406.

Suggested Citation

  • Rafiul Amin Laskar & Samiullah Khan, 2017. "Evaluation of Optimal Doses for Gamma Rays and Hydrazine Hydrates in Lentil Genotypes," Current Trends in Biomedical Engineering & Biosciences, Juniper Publishers Inc., vol. 8(1), pages 21-24, August.
    • Handle: RePEc:adp:jctbeb:v:8:y:2017:i:1:p:21-24
    DOI: 10.19080/CTBEB.2017.08.555730
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