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Identification and Analysis of Salt Responsive Candidate Gene Based on SSRs from Tomato (Solanum lycopersicum L.)

Author

Listed:
  • Jaafar Umar

    (Department of Biology, Federal University, Nigeria)

  • Aliero Adamu Aliyu
  • Shehu Kasimu

    (Department of Biological Sciences, Usmanu Danfodiyo University, Nigeria)

  • Abubakar Lawal

    (Department of Crop Science, Usmanu Danfodiyo University, Nigeria)

Abstract

Salinity inhibition of plant growth is the result of osmotic and ionic effect and different plant species have developed different mechanisms to cope with those effects. With the discovery of molecular markers and marker assisted selection technology, it is possible to develop markers that identify salt tolerance. This study aimed at identification salinity responsive gene based SSRs. In this study, genetic diversity of twenty tomato genotypes (landraces/accessions in Nigeria) DNA of the 20 accessions were isolated using Bioland Plant Genomic DNA protocols, Primers were designed from 15 different salt responsive candidate genes. All 15 primers sets generated shows clear distinct polymorphic profiles as evident from the 6% agarose gel profile. Data obtained were analyzed using SSRs polymorphic markers and Unweighted Pair Group Method with Arithmetic Mean.

Suggested Citation

  • Jaafar Umar & Aliero Adamu Aliyu & Shehu Kasimu & Abubakar Lawal, 2018. "Identification and Analysis of Salt Responsive Candidate Gene Based on SSRs from Tomato (Solanum lycopersicum L.)," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 5(1), pages 10-16, August.
    • Handle: RePEc:adp:ijcsmb:v:5:y:2018:i:1:p:10-16
    DOI: 10.19080/IJCSMB.2018.04.555653
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