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Studies Oniridovirus Infection in Grouper Fish (EpinephelusSp.) In Seribu Islands, Indonesia

Author

Listed:
  • NurulSulfi Andini

    (Gadjah Mada University, Indonesia)

  • Kurniasih

    (Pathology Department, Gadjah Mada University, Indonesia)

  • Tri Untari

    (Microbiology Department, Gadjah Mada University, Indonesia)

Abstract

Grouper fish has a high interest aquaculture in Indonesia, Singapore, Taiwan and China. Iridovirus infection can cause mass mortality in groupers. The outbreak of Iridovirus disease was often found in several marine aquacultures in islands of Indonesia, including Seribu Islands.The aim of research is to diagnose Iridovirus infection in grouper fish in Seribu Islands based on Polymerase Chain Reaction and immunehistochemistry methods. The number of 18 grouper fish was collected from the location of outbreak. Spleen was treated with Polymerase Chain Reaction method. Spleen, kidney, liver, gonad and gill were processed for all results were analyzed descriptively. Results of this research show 3 samples from Tidung Island was infected by Iridovirus and 15 samples showed negative result with PCR test. Result of immunohistochemistry tests of three positive samples were the presence of antigen-antibody reaction in several organ such as spleen, liver, gill, gonade, and eye. The inclution body was brown color within the cells.

Suggested Citation

  • NurulSulfi Andini & Kurniasih & Tri Untari, 2018. "Studies Oniridovirus Infection in Grouper Fish (EpinephelusSp.) In Seribu Islands, Indonesia," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 4(5), pages 95-98, May.
    • Handle: RePEc:adp:ijcsmb:v:4:y:2018:i:5:p:95-98
    DOI: 10.19080/IJCSMB.2018.03.555647
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