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Pathogenecity of Pseudomonas anguilliseptica Infection in Goldfish (Cyprinus Carpio)

Author

Listed:
  • AjengAekanurmaningdyah

    (Clinical Department, Brawijaya University, Indonesia)

  • Kurniasih

    (Pathology Department, Gadjah Mada University, Indonesia)

Abstract

Pseudomonas anguilliseptica caused Red Spot Disease (Sekitten - Byo) resulted a very high mortality in fishes and serious economic losses as a Quarantine Fish and Diseases group I.Aim of study is to identify Ps. anguilliseptica with conventional, molecular, and the histopathological changes. Local isolate of Ps. anguilliseptica were identified by morphological and molecular tests. Molecular identification in 16S rRNA fragment used forward primer Psan-F (21-mer:5’- TTGGGAGGAAGGGCA-GTAACC-3’) and reverse Psan-R(20-mer: 5’- TGCGCCACTAAAATCTCAAG-3’), and sequencing. Pathogenicity test used 18 fishes divided into 2 groups each groupconsist of 9 fishes. Group I, fish was infected intramuscularly with buffer saline as a control and group II, fish was infected intramuscularly with 0.1ml LC-50 of Ps. anguilliseptica suspension. Fishes were autopsied on 1st day,8th day and 15th day after infection.

Suggested Citation

  • AjengAekanurmaningdyah & Kurniasih, 2018. "Pathogenecity of Pseudomonas anguilliseptica Infection in Goldfish (Cyprinus Carpio)," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 4(5), pages 111-116, July.
    • Handle: RePEc:adp:ijcsmb:v:4:y:2018:i:5:p:111-116
    DOI: 10.19080/IJCSMB.2018.03.555650
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