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Effect of 2,4-dichlorophenoxyacetic on in Vitro Callus Induction Using Leaf of Rocoto (Capsicum Pubescens Ruiz & Pav.) Cv. Serrano

Author

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  • Hernández Amasifuen Angel David Author-Workplace- Department of Plant Biotechnology Laboratory of the Professional School of Biology with a mention in Biotechnology, José Faustino Sánchez Carrión National University, Peru
  • Argüelles Curaca Alexis, Cortez Lázaro Anthony Apolinario Author-Workplace- Department of Plant Biotechnology Laboratory of the Professional School of Biology with a mention in Biotechnology, José Faustino Sánchez Carrión National University, Peru
  • Barreto Romero Andrea Alhely Author-Workplace- Department of Plant Biotechnology Laboratory of the Professional School of Biology with a mention in Biotechnology, José Faustino Sánchez Carrión National University, Peru
  • Díaz Pillasca Hermila Belba Author-Workplace- Department of Plant Biotechnology Laboratory of the Professional School of Biology with a mention in Biotechnology, José Faustino Sánchez Carrión National University, Peru
  • Silva Vergara Mishell Zaori Author-Workplace- Department of Plant Biotechnology Laboratory of the Professional School of Biology with a mention in Biotechnology, José Faustino Sánchez Carrión National University, Peru

Abstract

The rocoto (Capsicum pubescen Ruiz & Pav.) is a plant of the genus Capsicum with center of origen in Perú, Used in national cuasina and of great valué for its pharmaceutical and medicinal propretores, relatad to capsaicin. In the present work a methodology was developed to determine the effect of 2,4-dichlorophenoxyacetic on in vitro callus induction from leaves in rocoto cv. Serrano. Were used seedlings rocoto cv. Serrano germínate in vitro, and subsequently the youngest leaves were selected, which were sectioned and placed in the different culture media. Five treatments with different concentrations of 2,4-D were evaluated. The best results were obtained with treatments T4 and T5 for the callus induction in 21 days allowed 72% callus proliferation.

Suggested Citation

  • Hernández Amasifuen Angel David Author-Workplace- Department of Plant Biotechnology Laboratory of the Professional School of Biology with a mention in Biotechnology, José Faustino Sánchez Carrión , 2019. "Effect of 2,4-dichlorophenoxyacetic on in Vitro Callus Induction Using Leaf of Rocoto (Capsicum Pubescens Ruiz & Pav.) Cv. Serrano," Agricultural Research & Technology: Open Access Journal, Juniper Publishers Inc., vol. 23(3), pages 280-284, October.
    • Handle: RePEc:adp:artoaj:v:23:y:2019:i:3:p:280-284
    DOI: 10.19080/ARTOAJ.2019.23.5556229
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