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Effect of Agar Weight Loss with Continuous Active Air Monitoring According GMP Annex 1 2023

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  • Roberto Ligugnana

    (SIMA Italian Society for Applied Microbiology, Veterinary Facultat – Università degli Studi Milano, Istituto Ispezione Alimenti Origine Animale, Italy)

Abstract

The GMP Annex 1 2023 requests that microbial environmental monitoring by active microbial air samplers is actuated by continuous air monitoring. A prolonged sampling produces a loss of moisture in the agar of the Petri dish and therefore it is necessary that during the “continuous time†the dryness of the nutrient media is under control to guarantee a regular growing of the CFU. The purpose of this paper is to evaluate if the agar (which volume was reduced with a treatment under sterile laminar flow before aspiration) has still the correct nutrient characteristics during the incubation of the Petri dishes. The tested instrument was a AIR. BIO ONE air sampler with an aspiration rate of 25 liters / minute. The results of the study are summarized in (Tables 1 & 2).

Suggested Citation

  • Roberto Ligugnana, 2023. "Effect of Agar Weight Loss with Continuous Active Air Monitoring According GMP Annex 1 2023," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 53(3), pages 44823-44825, October.
    • Handle: RePEc:abf:journl:v:53:y:2023:i:3:p:44823-44825
    DOI: 10.26717/BJSTR.2023.53.008415
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