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Comparison of Four Electrophoresis Buffer for Good Performance as DNA Separator on Agarose Gel

Author

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  • Sahar Abd Al-Daim

    (Water Pollution Research Department, Environment and Climate Change Institute, National Research Centre, Egypt)

Abstract

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this we used two agarose concentration (1%, 2%) and two types of DNA ladder (100bp, 1kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.

Suggested Citation

  • Sahar Abd Al-Daim, 2023. "Comparison of Four Electrophoresis Buffer for Good Performance as DNA Separator on Agarose Gel," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 49(4), pages 40858-40862, April.
    • Handle: RePEc:abf:journl:v:49:y:2023:i:4:p:40858-40862
    DOI: 10.26717/BJSTR.2023.49.007829
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