IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v13y2020i10p2588-d360357.html
   My bibliography  Save this article

Biodiesel Production by Lipase-Catalyzed in Situ Transesterification of Rapeseed Oil Containing a High Free Fatty Acid Content with Ethanol in Diesel Fuel Media

Author

Listed:
  • Migle Santaraite

    (Faculty of Forest Sciences and Ecology, Agriculture Academy, Vytautas Magnus University, LT-44248 Kaunas, Lithuania)

  • Egle Sendzikiene

    (Faculty of Forest Sciences and Ecology, Agriculture Academy, Vytautas Magnus University, LT-44248 Kaunas, Lithuania)

  • Violeta Makareviciene

    (Faculty of Forest Sciences and Ecology, Agriculture Academy, Vytautas Magnus University, LT-44248 Kaunas, Lithuania)

  • Kiril Kazancev

    (Faculty of Forest Sciences and Ecology, Agriculture Academy, Vytautas Magnus University, LT-44248 Kaunas, Lithuania)

Abstract

In this study, low-quality rapeseed was used as a raw material for biodiesel fuel production. The application of such seeds with an enzyme catalyst is a green approach to producing renewable biodiesel fuel. During the in situ transesterification process, mineral diesel was selected as an extraction solvent for the simultaneous extraction and transesterification of rapeseed oil (RO). This allowed, at the end of the process, for the production of a mixture of mineral diesel and biodiesel fuel. Energy is saved using this process, as the need to extract the oil separately is eliminated and extraction and transesterification take place together in the in situ process. In this study, 11 different lipases were analyzed from which to select the most effective biocatalyst according to the chosen experimental conditions. The most suitable lipase for in situ transesterification was Lipozyme TL IM (Thermomyces lanuginosus). The impact of the temperature and duration of the reaction was investigated along with the concentration of the lipase. A ethanol-to-oil molar ratio of 5:1 was chosen. The optimal reaction conditions were as follows: a reaction duration of 7 h, a reaction temperature of 30 °C and a lipase concentration of 5% (based on oil weight). Under these conditions, 99.92% of oil was extracted from the rapeseed. The degree of oil transesterification acquired was 99.89%. A mineral diesel and rapeseed oil ethyl ester blend of 9:1 (w/w) was produced.

Suggested Citation

  • Migle Santaraite & Egle Sendzikiene & Violeta Makareviciene & Kiril Kazancev, 2020. "Biodiesel Production by Lipase-Catalyzed in Situ Transesterification of Rapeseed Oil Containing a High Free Fatty Acid Content with Ethanol in Diesel Fuel Media," Energies, MDPI, vol. 13(10), pages 1-12, May.
    • Handle: RePEc:gam:jeners:v:13:y:2020:i:10:p:2588-:d:360357
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/13/10/2588/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/13/10/2588/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Violeta Makareviciene & Egle Sendzikiene & Milda Gumbyte, 2020. "Application of Simultaneous Oil Extraction and Transesterification in Biodiesel Fuel Synthesis: A Review," Energies, MDPI, vol. 13(9), pages 1-16, May.
    2. Zhao, Xuebing & Qi, Feng & Yuan, Chongli & Du, Wei & Liu, Dehua, 2015. "Lipase-catalyzed process for biodiesel production: Enzyme immobilization, process simulation and optimization," Renewable and Sustainable Energy Reviews, Elsevier, vol. 44(C), pages 182-197.
    3. Abo El-Enin, S.A. & Attia, N.K. & El-Ibiari, N.N. & El-Diwani, G.I. & El-Khatib, K.M., 2013. "In-situ transesterification of rapeseed and cost indicators for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 18(C), pages 471-477.
    4. Go, Alchris Woo & Sutanto, Sylviana & Ong, Lu Ki & Tran-Nguyen, Phuong Lan & Ismadji, Suryadi & Ju, Yi-Hsu, 2016. "Developments in in-situ (trans) esterification for biodiesel production: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 60(C), pages 284-305.
    5. Bolonio, David & García-Martínez, María-Jesús & Ortega, Marcelo F. & Lapuerta, Magín & Rodríguez-Fernández, José & Canoira, Laureano, 2019. "Fatty acid ethyl esters (FAEEs) obtained from grapeseed oil: A fully renewable biofuel," Renewable Energy, Elsevier, vol. 132(C), pages 278-283.
    6. Szczęsna Antczak, Mirosława & Kubiak, Aneta & Antczak, Tadeusz & Bielecki, Stanisław, 2009. "Enzymatic biodiesel synthesis – Key factors affecting efficiency of the process," Renewable Energy, Elsevier, vol. 34(5), pages 1185-1194.
    7. No, Soo-Young, 2011. "Inedible vegetable oils and their derivatives for alternative diesel fuels in CI engines: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(1), pages 131-149, January.
    8. Patel, Rupesh L. & Sankhavara, C.D., 2017. "Biodiesel production from Karanja oil and its use in diesel engine: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 71(C), pages 464-474.
    9. Avhad, M.R. & Marchetti, J.M., 2015. "A review on recent advancement in catalytic materials for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 50(C), pages 696-718.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Violeta Makareviciene, 2022. "Advances in Catalytic Technologies for Biodiesel Fuel Synthesis," Energies, MDPI, vol. 15(3), pages 1-4, January.
    2. Violeta Makareviciene & Kiril Kazancev & Egle Sendzikiene & Milda Gumbyte, 2024. "Enzymatic In Situ Interesterification of Rapeseed Oil with Methyl Formate in Diesel Fuel Medium," Energies, MDPI, vol. 17(2), pages 1-13, January.
    3. Egle Sendzikiene & Violeta Makareviciene & Migle Santaraite, 2022. "Simultaneous Extraction of Rapeseed Oil and Enzymatic Transesterification with Butanol in the Mineral Diesel Medium," Energies, MDPI, vol. 15(18), pages 1-12, September.
    4. Zulfiqar, Anam & Mumtaz, Muhammad Waseem & Mukhtar, Hamid & Najeeb, Jawayria & Irfan, Ahmad & Akram, Sadia & Touqeer, Tooba & Nabi, Ghulam, 2021. "Lipase-PDA-TiO2 NPs: An emphatic nano-biocatalyst for optimized biodiesel production from Jatropha curcas oil," Renewable Energy, Elsevier, vol. 169(C), pages 1026-1037.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Tacias-Pascacio, Veymar G. & Torrestiana-Sánchez, Beatriz & Dal Magro, Lucas & Virgen-Ortíz, Jose J. & Suárez-Ruíz, Francisco J. & Rodrigues, Rafael C. & Fernandez-Lafuente, Roberto, 2019. "Comparison of acid, basic and enzymatic catalysis on the production of biodiesel after RSM optimization," Renewable Energy, Elsevier, vol. 135(C), pages 1-9.
    2. Pourzolfaghar, Hamed & Abnisa, Faisal & Daud, Wan Mohd Ashri Wan & Aroua, Mohamed Kheireddine, 2016. "A review of the enzymatic hydroesterification process for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 61(C), pages 245-257.
    3. Aguieiras, Erika C.G. & de Barros, Daniele S.N. & Fernandez-Lafuente, Roberto & Freire, Denise M.G., 2019. "Production of lipases in cottonseed meal and application of the fermented solid as biocatalyst in esterification and transesterification reactions," Renewable Energy, Elsevier, vol. 130(C), pages 574-581.
    4. Zhang, Zhijin & Du, Yingjie & Kuang, Geling & Shen, Xuejian & Jia, Xiaotong & Wang, Ziyuan & Feng, Yuxiao & Jia, Shiru & Liu, Fufeng & Bilal, Muhammad & Cui, Jiandong, 2022. "Lipase-Ca2+ hybrid nanobiocatalysts through interfacial protein-inorganic self-assembly in deep-eutectic solvents (DES)/water two-phase system for biodiesel production," Renewable Energy, Elsevier, vol. 197(C), pages 110-124.
    5. Zhong, Le & Jiao, Xiaobo & Hu, Hongtong & Shen, Xuejian & Zhao, Juan & Feng, Yuxiao & Li, Conghai & Du, Yingjie & Cui, Jiandong & Jia, Shiru, 2021. "Activated magnetic lipase-inorganic hybrid nanoflowers: A highly active and recyclable nanobiocatalyst for biodiesel production," Renewable Energy, Elsevier, vol. 171(C), pages 825-832.
    6. Silvia Cesarini & F. I. Javier Pastor & Per M. Nielsen & Pilar Diaz, 2015. "Moving towards a Competitive Fully Enzymatic Biodiesel Process," Sustainability, MDPI, vol. 7(6), pages 1-20, June.
    7. Samuel Santos & Jaime Puna & João Gomes, 2020. "A Review on Bio-Based Catalysts (Immobilized Enzymes) Used for Biodiesel Production," Energies, MDPI, vol. 13(11), pages 1-19, June.
    8. Egle Sendzikiene & Violeta Makareviciene & Migle Santaraite, 2022. "Simultaneous Extraction of Rapeseed Oil and Enzymatic Transesterification with Butanol in the Mineral Diesel Medium," Energies, MDPI, vol. 15(18), pages 1-12, September.
    9. Arumugam, A. & Thulasidharan, D. & Jegadeesan, Gautham B., 2018. "Process optimization of biodiesel production from Hevea brasiliensis oil using lipase immobilized on spherical silica aerogel," Renewable Energy, Elsevier, vol. 116(PA), pages 755-761.
    10. Ewunie, Gebresilassie Asnake & Morken, John & Lekang, Odd Ivar & Yigezu, Zerihun Demrew, 2021. "Factors affecting the potential of Jatropha curcas for sustainable biodiesel production: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 137(C).
    11. Sitepu, Eko K. & Heimann, Kirsten & Raston, Colin L. & Zhang, Wei, 2020. "Critical evaluation of process parameters for direct biodiesel production from diverse feedstock," Renewable and Sustainable Energy Reviews, Elsevier, vol. 123(C).
    12. Sajjadi, Baharak & Raman, Abdul Aziz Abdul & Arandiyan, Hamidreza, 2016. "A comprehensive review on properties of edible and non-edible vegetable oil-based biodiesel: Composition, specifications and prediction models," Renewable and Sustainable Energy Reviews, Elsevier, vol. 63(C), pages 62-92.
    13. Christopher, Lew P. & Hemanathan Kumar, & Zambare, Vasudeo P., 2014. "Enzymatic biodiesel: Challenges and opportunities," Applied Energy, Elsevier, vol. 119(C), pages 497-520.
    14. Mansir, Nasar & Teo, Siow Hwa & Rashid, Umer & Saiman, Mohd Izham & Tan, Yen Ping & Alsultan, G. Abdulkareem & Taufiq-Yap, Yun Hin, 2018. "Modified waste egg shell derived bifunctional catalyst for biodiesel production from high FFA waste cooking oil. A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 82(P3), pages 3645-3655.
    15. di Bitonto, Luigi & Lopez, Antonio & Mascolo, Giuseppe & Mininni, Giuseppe & Pastore, Carlo, 2016. "Efficient solvent-less separation of lipids from municipal wet sewage scum and their sustainable conversion into biodiesel," Renewable Energy, Elsevier, vol. 90(C), pages 55-61.
    16. Patchimpet, Jaran & Simpson, Benjamin K. & Sangkharak, Kanokphorn & Klomklao, Sappasith, 2020. "Optimization of process variables for the production of biodiesel by transesterification of used cooking oil using lipase from Nile tilapia viscera," Renewable Energy, Elsevier, vol. 153(C), pages 861-869.
    17. Asokan, M.A. & Senthur Prabu, S. & Bade, Pushpa Kiran Kumar & Nekkanti, Venkata Mukesh & Gutta, Sri Sai Gopal, 2019. "Performance, combustion and emission characteristics of juliflora biodiesel fuelled DI diesel engine," Energy, Elsevier, vol. 173(C), pages 883-892.
    18. Zahedi, Ali Reza & Mirnezami, Seyed Abolfazl, 2020. "Experimental analysis of biomass to biodiesel conversion using a novel renewable combined cycle system," Renewable Energy, Elsevier, vol. 162(C), pages 1177-1194.
    19. Vela-García, Nicolas & Bolonio, David & Mosquera, Ana María & Ortega, Marcelo F. & García-Martínez, María-Jesús & Canoira, Laureano, 2020. "Techno-economic and life cycle assessment of triisobutane production and its suitability as biojet fuel," Applied Energy, Elsevier, vol. 268(C).
    20. Ramalingam, Senthil & Rajendran, Silambarasan & Ganesan, Pranesh & Govindasamy, Mohan, 2018. "Effect of operating parameters and antioxidant additives with biodiesels to improve the performance and reducing the emissions in a compression ignition engine – A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 81(P1), pages 775-788.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:13:y:2020:i:10:p:2588-:d:360357. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.