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Exchanging and managing in-vitro elite germplasm to combat Cassava Brown Streak Disease (CBSD) and Cassava Mosaic Disease (CMD) in Eastern and Southern Africa

Author

Listed:
  • Silver Tumwegamire

    (International Institute of Tropical Agriculture (IITA))

  • Edward Kanju

    (International Institute of Tropical Agriculture (IITA))

  • James Legg

    (International Institute of Tropical Agriculture (IITA))

  • Rudolph Shirima

    (International Institute of Tropical Agriculture (IITA))

  • Salehe Kombo

    (International Institute of Tropical Agriculture (IITA))

  • Geoffrey Mkamilo

    (Department of Agricultural Research and Development)

  • Kiddo Mtunda

    (Department of Agricultural Research and Development)

  • Karoline Sichalwe

    (Department of Agricultural Research and Development)

  • Heneriko Kulembeka

    (Department of Agricultural Research and Development)

  • Innocent Ndyetabura

    (Department of Agricultural Research and Development)

  • Haji Saleh

    (Zanzibar Agricultural Research Institute)

  • Robert Kawuki

    (National Agricultural Research Organization)

  • Titus Alicai

    (National Agricultural Research Organization)

  • Gerald Adiga

    (National Agricultural Research Organization)

  • Ibrahim Benesi

    (Chitedze Research Station)

  • Albert Mhone

    (Chitedze Research Station)

  • Anabela Zacarias

    (Instituto de Investigacao Agraria de Mocambique)

  • Sofrimento Fenias Matsimbe

    (Instituto de Investigacao Agraria de Mocambique)

  • Theresia Munga

    (Kenya Agricultural and Livestock Research Organization)

  • Elijah Ateka

    (Jomo Kenyatta University of Agriculture and Technology)

  • Lynet Navangi

    (Kenya Agricultural and Livestock Research Organization)

  • Midatharahally Narasegowda Maruthi

    (University of Greenwich)

  • Francis Mwatuni

    (Plant Quarantine and Biosecurity Station)

  • George Ngundo

    (Plant Quarantine and Biosecurity Station)

  • Maureen Mwangangi

    (Plant Quarantine and Biosecurity Station)

  • Edward Mbugua

    (Genetic Technologies International Limited)

  • Joseph Ndunguru

    (Mikocheni Agricultural Research Institute)

  • Cyprian Rajabu

    (Mikocheni Agricultural Research Institute)

  • Deogratius Mark

    (Mikocheni Agricultural Research Institute)

Abstract

Cassava varieties resistant to cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) are needed for the food and income security of the rural poor in eastern and southern Africa (ESA). The International Institute of Tropical Agriculture led five national cassava breeding programs (Malawi, Mozambique, Kenya, Tanzania and Uganda) in virus-cleaning and exchanging elite cassava germplasm resistant to both diseases. This paper documents the experiences and lessons learned from the process. Thirty-one clones (25 elite, two standard and four national) were submitted by the five breeding programs to the Natural Resources Institute and Kenya Plant Health Inspectorate Services for virus cleaning and indexing. Subsequently, ca 75 in-vitro virus-indexed plantlets per clone were sent to Genetic Technologies International Limited (GTIL), a private tissue culture (TC) lab in Kenya, and micro-propagated to produce ≥1500 plantlets. After fulfilling all the formal procedures of germplasm exchange between countries ≥300 plantlets per clone were sent to each partner country. National check clones susceptible to CMD/CBSD were sent only to their countries of origin. In each country, the in-vitro plantlets were acclimatized under screen house conditions and transferred to clean isolated sites for field multiplication. All the clones were cleaned of the viruses, except Tomo. The cleaning process was slow for F19-NL, NASE1, and Kibandameno and TC micro-propagation at GTIL was less efficient for Pwani, Tajirika, NASE1, and Okhumelela than for the other clones. Difficulties in cleaning recalcitrant clones affected the timeline for establishing the multi-site evaluation trials in target countries. The initiative is the one of the kind to successfully clean and exchange elite germplasm as a joint action to combat CBSD in ESA. Adequate preparation in terms of infrastructure and personnel are critical to successfully receiving and adapting the indexed in-vitro plants as new germplasm.

Suggested Citation

  • Silver Tumwegamire & Edward Kanju & James Legg & Rudolph Shirima & Salehe Kombo & Geoffrey Mkamilo & Kiddo Mtunda & Karoline Sichalwe & Heneriko Kulembeka & Innocent Ndyetabura & Haji Saleh & Robert K, 2018. "Exchanging and managing in-vitro elite germplasm to combat Cassava Brown Streak Disease (CBSD) and Cassava Mosaic Disease (CMD) in Eastern and Southern Africa," Food Security: The Science, Sociology and Economics of Food Production and Access to Food, Springer;The International Society for Plant Pathology, vol. 10(2), pages 351-368, April.
  • Handle: RePEc:spr:ssefpa:v:10:y:2018:i:2:d:10.1007_s12571-018-0779-2
    DOI: 10.1007/s12571-018-0779-2
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