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Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomics

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  • Wenjin Hu
  • Xinli Pan
  • Hafiz Muhammad Khalid Abbas
  • Fengfeng Li
  • Wubei Dong

Abstract

Rhizoctonia solani is a causative agent of sheath blight, which results in huge economic losses every year. During its life cycle, the formation of sclerotia helps Rhizoctonia solani withstand a variety of unfavorable factors. Oxidative stress is a key factor that induces sclerotium formation. The differentiated and undifferentiated phenotypes of R. solani AG-1-IA were obtained by controlling aerial conditions. Metabolomics based on the mass spectrometry technique combined with multivariate and univariate analyses was used to investigate the metabolic variation in vegetative, differentiated and undifferentiated mycelia. Our results revealed that during maturation, the metabolic levels of N2-acetyl-L-ornithine, 3,1'-(OH)2-Gamma-carotene, (5Z,7E)-(1S,3R)-24,24-difluoro-24a-homo-9,10-seco-5,7,10(19)-cholestatrien-1,3,25-triol, stoloniferone O, PA(O-18:0/12:0), PA(P-16:0/14:0), PA(P-16:0/16:(19Z)) and PA(P-16:0/17:2(9Z,12Z)) were suppressed in both differentiated and undifferentiated mycelia. The concentrations of PE(20:1(11Z)/14:1(9Z)), PE(P-16:0/20:4(5Z,8Z,11Z,13E)(15OH[S])) and PS(12:0/18:1(9Z)) were increased in the differentiated group, while increased levels of N(gamma)-nitro-L-arginine, tenuazonic acid and 9S,10S,11R-trihydroxy-12Z,15Z-octadecadienoic acid were found in the undifferentiated group. Our results suggest that different levels of these metabolites may act as biomarkers for the developmental stages of R. solani AG-1-IA. Moreover, the mechanisms of sclerotium formation and mycelium differentiation were elucidated at the metabolic level.

Suggested Citation

  • Wenjin Hu & Xinli Pan & Hafiz Muhammad Khalid Abbas & Fengfeng Li & Wubei Dong, 2017. "Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomics," PLOS ONE, Public Library of Science, vol. 12(5), pages 1-16, May.
    • Handle: RePEc:plo:pone00:0177464
    DOI: 10.1371/journal.pone.0177464
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    References listed on IDEAS

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    1. Choong-Soo Yun & Takayuki Motoyama & Hiroyuki Osada, 2015. "Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS–PKS hybrid enzyme," Nature Communications, Nature, vol. 6(1), pages 1-9, December.
    2. Aiping Zheng & Runmao Lin & Danhua Zhang & Peigang Qin & Lizhi Xu & Peng Ai & Lei Ding & Yanran Wang & Yao Chen & Yao Liu & Zhigang Sun & Haitao Feng & Xiaoxing Liang & Rongtao Fu & Changqing Tang & Q, 2013. "The evolution and pathogenic mechanisms of the rice sheath blight pathogen," Nature Communications, Nature, vol. 4(1), pages 1-10, June.
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    1. Wenjin Hu & Xinli Pan & Fengfeng Li & Wubei Dong, 2018. "UPLC-QTOF-MS metabolomics analysis revealed the contributions of metabolites to the pathogenesis of Rhizoctonia solani strain AG-1-IA," PLOS ONE, Public Library of Science, vol. 13(2), pages 1-17, February.
    2. Yueping Zhu & Qilai Xie & Jinshao Ye & Ruzhen Wang & Xudong Yin & Wenyu Xie & Dehao Li, 2023. "Metabolic Mechanism of Bacillus sp. LM24 under Abamectin Stress," IJERPH, MDPI, vol. 20(4), pages 1-24, February.

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