The impact of interactions on invasion and colonization resistance in microbial communities

In human microbiota, the prevention or promotion of invasions can be crucial to human health. Invasion outcomes, in turn, are impacted by the composition of resident communities and interactions of resident members with the invader. Here we study how interactions influence invasion outcomes in microbial communities, when interactions are primarily mediated by chemicals that are released into or consumed from the environment. We use a previously developed dynamic model which explicitly includes species abundances and the concentrations of chemicals that mediate species interaction. Using this model, we assessed how species interactions impact invasion by simulating a new species being introduced into an existing resident community. We classified invasion outcomes as resistance, augmentation, displacement, or disruption depending on whether the richness of the resident community was maintained or decreased and whether the invader was maintained in the community or went extinct. We found that as the number of invaders introduced into the resident community increased, disruption rather than augmentation became more prevalent. With more facilitation of the invader by the resident community, resistance outcomes were replaced by displacement and augmentation. By contrast, with more facilitation among residents, displacement outcomes shifted to resistance. When facilitation of the resident community by the invader was eliminated, the majority of augmentation outcomes turned into displacement, while when inhibition of residents by invaders was eliminated, invasion outcomes were largely unaffected. Our results suggest that a better understanding of interactions within resident communities and between residents and invaders is crucial to predicting the success of invasions into microbial communities.Author summary: Our resident microbiota can prevent diseases by making it harder for pathogens to grow and establish, a phenomenon called “colonization resistance.” Colonization resistance is one of the major benefits provided by human-associated microbiota and a viable alternative to the use of antibiotics for preventing or treating infections. Here we use a model of microbial interactions through production and consumption of metabolic compounds to assay invasion and colonization resistance. We systematically examine in simulations how interactions among resident members and those between residents and an invader impact colonization resistance and invasion outcomes. In our simulations, the common strategy of increasing the dosage of probiotics is often unsuccessful for augmenting a new species into a resident microbiota. Instead, we find that the net facilitation or inhibition between the resident members and the invader explains whether the community remains intact and whether the invader can establish. Our results suggest that a better understanding of microbial interactions can inform successful microbiota interventions.