IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms13308.html
   My bibliography  Save this article

Structure of the bacterial plant-ferredoxin receptor FusA

Author

Listed:
  • Rhys Grinter

    (Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow
    Institute of Microbiology and Infection, School of Immunity and Infection, University of Birmingham
    Infection and Immunity Program, Monash University)

  • Inokentijs Josts

    (Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Khedidja Mosbahi

    (Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Aleksander W. Roszak

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Richard J. Cogdell

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Alexandre M. J. J. Bonvin

    (Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University)

  • Joel J. Milner

    (School of Life Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Sharon M. Kelly

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Olwyn Byron

    (School of Life Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Brian O. Smith

    (Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow)

  • Daniel Walker

    (Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow)

Abstract

Iron is a limiting nutrient in bacterial infection putting it at the centre of an evolutionary arms race between host and pathogen. Gram-negative bacteria utilize TonB-dependent outer membrane receptors to obtain iron during infection. These receptors acquire iron either in concert with soluble iron-scavenging siderophores or through direct interaction and extraction from host proteins. Characterization of these receptors provides invaluable insight into pathogenesis. However, only a subset of virulence-related TonB-dependent receptors have been currently described. Here we report the discovery of FusA, a new class of TonB-dependent receptor, which is utilized by phytopathogenic Pectobacterium spp. to obtain iron from plant ferredoxin. Through the crystal structure of FusA we show that binding of ferredoxin occurs through specialized extracellular loops that form extensive interactions with ferredoxin. The function of FusA and the presence of homologues in clinically important pathogens suggests that small iron-containing proteins represent an iron source for bacterial pathogens.

Suggested Citation

  • Rhys Grinter & Inokentijs Josts & Khedidja Mosbahi & Aleksander W. Roszak & Richard J. Cogdell & Alexandre M. J. J. Bonvin & Joel J. Milner & Sharon M. Kelly & Olwyn Byron & Brian O. Smith & Daniel Wa, 2016. "Structure of the bacterial plant-ferredoxin receptor FusA," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13308
    DOI: 10.1038/ncomms13308
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms13308
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms13308?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Rhys Grinter & Iain D Hay & Jiangning Song & Jiawei Wang & Don Teng & Vijay Dhanesakaran & Jonathan J Wilksch & Mark R Davies & Dene Littler & Simone A Beckham & Ian R Henderson & Richard A Strugnell , 2018. "FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants," PLOS Biology, Public Library of Science, vol. 16(8), pages 1-21, August.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13308. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.