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LWD–TCP complex activates the morning gene CCA1 in Arabidopsis

Author

Listed:
  • Jing-Fen Wu

    (Institute of Plant and Microbial Biology, Academia Sinica,)

  • Huang-Lung Tsai

    (Institute of Plant and Microbial Biology, Academia Sinica,)

  • Ignasius Joanito

    (Institute of Chemistry, Academia Sinica
    National Chiao Tung University
    Bioinformatics Program, Taiwan International Graduate Program, Academia Sinica
    Institute of Bioinformatics and System Biology, National Chiao Tung University)

  • Yi-Chen Wu

    (Institute of Plant and Microbial Biology, Academia Sinica,)

  • Chin-Wen Chang

    (Institute of Plant and Microbial Biology, Academia Sinica,)

  • Yi-Hang Li

    (Institute of Plant and Microbial Biology, Academia Sinica,)

  • Ying Wang

    (Institute of Plant and Microbial Biology, Academia Sinica,)

  • Jong Chan Hong

    (Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University
    University of Missouri)

  • Jhih-Wei Chu

    (National Chiao Tung University
    Bioinformatics Program, Taiwan International Graduate Program, Academia Sinica
    Institute of Bioinformatics and System Biology, National Chiao Tung University)

  • Chao-Ping Hsu

    (Institute of Chemistry, Academia Sinica
    Genome and Systems Biology Degree Program, National Taiwan University)

  • Shu-Hsing Wu

    (Institute of Plant and Microbial Biology, Academia Sinica,
    Genome and Systems Biology Degree Program, National Taiwan University)

Abstract

A double-negative feedback loop formed by the morning genes CIRCADIAN CLOCK ASSOCIATED1 (CCA1)/LATE ELONGATED HYPOCOTYL (LHY) and the evening gene TIMING OF CAB EXPRESSION1 (TOC1) contributes to regulation of the circadian clock in Arabidopsis. A 24-h circadian cycle starts with the peak expression of CCA1 at dawn. Although CCA1 is targeted by multiple transcriptional repressors, including PSEUDO-RESPONSE REGULATOR9 (PRR9), PRR7, PRR5 and CCA1 HIKING EXPEDITION (CHE), activators of CCA1 remain elusive. Here we use mathematical modelling to infer a co-activator role for LIGHT-REGULATED WD1 (LWD1) in CCA1 expression. We show that the TEOSINTE BRANCHED 1-CYCLOIDEA-PCF20 (TCP20) and TCP22 proteins act as LWD-interacting transcriptional activators. The concomitant binding of LWD1 and TCP20/TCP22 to the TCP-binding site in the CCA1 promoter activates CCA1. Our study reveals activators of the morning gene CCA1 and provides an action mechanism that ensures elevated expression of CCA1 at dawn to sustain a robust clock.

Suggested Citation

  • Jing-Fen Wu & Huang-Lung Tsai & Ignasius Joanito & Yi-Chen Wu & Chin-Wen Chang & Yi-Hang Li & Ying Wang & Jong Chan Hong & Jhih-Wei Chu & Chao-Ping Hsu & Shu-Hsing Wu, 2016. "LWD–TCP complex activates the morning gene CCA1 in Arabidopsis," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13181
    DOI: 10.1038/ncomms13181
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    Cited by:

    1. Mengyao Fu & Yaoyu Zhou & Xin Zhang & Keyi Yang & Yufeng Xu & Xingwei Wang & Zhaodan Chen & Yu Wang & Yabo Shi & Lin Ma & Hanguang Liu & Yuhua Deng & Shujing Cheng & Jinfang Chu & Jingyi Song & Tongju, 2025. "A pathogen effector HaRxL10 hijacks the circadian clock component CHE to perturb both plant development and immunity," Nature Communications, Nature, vol. 16(1), pages 1-20, December.
    2. Weiliang Mo & Junchuan Zhang & Li Zhang & Zhenming Yang & Liang Yang & Nan Yao & Yong Xiao & Tianhong Li & Yaxing Li & Guangmei Zhang & Mingdi Bian & Xinglin Du & Zecheng Zuo, 2022. "Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clock," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Liang Cai & Benyuan Hao & Zhuang Xu & Song Cui & Qianyun Wu & Jiyoung Lee & Haigang Hou & Yuan Hu & Liang Zhu & Jie Wang & Wen Li & Kongyou Chang & Weihao Shao & Shanshan Zhu & Xiangchao Gan & Chao Li, 2025. "ELD1 mediates photoperiodic flowering via OsCCA1 alternative splicing and interacts with phytochrome signaling in rice," Nature Communications, Nature, vol. 16(1), pages 1-17, December.

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