IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms12404.html
   My bibliography  Save this article

PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2

Author

Listed:
  • Gabrielle J. Grundy

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Luis M. Polo

    (Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Zhihong Zeng

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Stuart L. Rulten

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Nicolas C. Hoch

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
    CAPES Foundation, Ministry of Education of Brazil)

  • Pathompong Paomephan

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Yingqi Xu

    (Cross-faculty NMR centre, Faculty of Natural Sciences, Imperial College London)

  • Steve M. Sweet

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Alan W. Thorne

    (Institute of Biomedical and Biomolecular Sciences, University of Portsmouth)

  • Antony W. Oliver

    (Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Steve J. Matthews

    (Cross-faculty NMR centre, Faculty of Natural Sciences, Imperial College London)

  • Laurence H. Pearl

    (Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • Keith W. Caldecott

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

Abstract

PARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break.

Suggested Citation

  • Gabrielle J. Grundy & Luis M. Polo & Zhihong Zeng & Stuart L. Rulten & Nicolas C. Hoch & Pathompong Paomephan & Yingqi Xu & Steve M. Sweet & Alan W. Thorne & Antony W. Oliver & Steve J. Matthews & Lau, 2016. "PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2," Nature Communications, Nature, vol. 7(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12404
    DOI: 10.1038/ncomms12404
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms12404
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms12404?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Zhen Huang & Ivanete De O. Furo & Jing Liu & Valentina Peona & Anderson J. B. Gomes & Wan Cen & Hao Huang & Yanding Zhang & Duo Chen & Ting Xue & Qiujin Zhang & Zhicao Yue & Quanxi Wang & Lingyu Yu & , 2022. "Recurrent chromosome reshuffling and the evolution of neo-sex chromosomes in parrots," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12404. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.