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Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

Author

Listed:
  • Fayza Daboussi

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Sophie Leduc

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Alan Maréchal

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Gwendoline Dubois

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Valérie Guyot

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Christophe Perez-Michaut

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Alberto Amato

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Angela Falciatore

    (Université Pierre et Marie Curie, UMR7238, CNRS-UPMC
    CNRS, UMR7238, Laboratoire Biologie Computationnelle et Quantitative)

  • Alexandre Juillerat

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Marine Beurdeley

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Daniel F. Voytas

    (Cellectis plant sciences)

  • Laurent Cavarec

    (Cellectis S.A., 8 rue de la Croix de Jarry)

  • Philippe Duchateau

    (Cellectis S.A., 8 rue de la Croix de Jarry)

Abstract

Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.

Suggested Citation

  • Fayza Daboussi & Sophie Leduc & Alan Maréchal & Gwendoline Dubois & Valérie Guyot & Christophe Perez-Michaut & Alberto Amato & Angela Falciatore & Alexandre Juillerat & Marine Beurdeley & Daniel F. Vo, 2014. "Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology," Nature Communications, Nature, vol. 5(1), pages 1-7, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4831
    DOI: 10.1038/ncomms4831
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    Cited by:

    1. Yanchun You & Xueqiong Sun & Minglei Ma & Jiamin He & Ling Li & Felipe Wendt Porto & Senjie Lin, 2022. "Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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