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The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication

Author

Listed:
  • Kyohei Matsuhashi

    (The University of Tokyo)

  • Kei K. Ito

    (The University of Tokyo)

  • Kaho Nagai

    (The University of Tokyo)

  • Akira Sanada

    (The University of Tokyo)

  • Koki Watanabe

    (The University of Tokyo
    EMBL Heidelberg)

  • Kasuga Takumi

    (The University of Tokyo)

  • Atsushi Toyoda

    (National Institute of Genetics)

  • Masamitsu Fukuyama

    (The University of Tokyo)

  • Shohei Yamamoto

    (The University of Tokyo)

  • Takumi Chinen

    (The University of Tokyo)

  • Grant S. Stewart

    (University of Birmingham)

  • Shoji Hata

    (The University of Tokyo)

  • Daiju Kitagawa

    (The University of Tokyo)

Abstract

Copy number control of DNA and centrosomes is essential for accurate genetic inheritance. DNA replication and centrosome duplication have been recognized as parallel key events for cell division. Here, we discover that the DNA replication machinery directly regulates the licensing and execution processes of centrosome duplication to prevent centrosome amplification. We find that the microcephaly protein DONSON couples DNA replication initiation with Cdc6 translocation to centrosomes. The Cdc6 signal prevents the precocious occurrence of centriole disengagement, the licensing step for centrosome duplication. During DNA replication, DONSON inhibits replisome disassembly by interacting with the CMG helicase, maintaining the intrinsic S/G2 checkpoint signal that blocks centriole-to-centrosome conversion, the execution step for centrosome duplication. Disruption of these dual signals causes precocious centrosome duplication and chromosome mis-segregation, observed in DONSON patient cells. Our results reveal that the DNA replication machinery not only duplicates genetic material but also controls the system for its accurate segregation.

Suggested Citation

  • Kyohei Matsuhashi & Kei K. Ito & Kaho Nagai & Akira Sanada & Koki Watanabe & Kasuga Takumi & Atsushi Toyoda & Masamitsu Fukuyama & Shohei Yamamoto & Takumi Chinen & Grant S. Stewart & Shoji Hata & Dai, 2025. "The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-63002-3
    DOI: 10.1038/s41467-025-63002-3
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    References listed on IDEAS

    as
    1. Josh Abramson & Jonas Adler & Jack Dunger & Richard Evans & Tim Green & Alexander Pritzel & Olaf Ronneberger & Lindsay Willmore & Andrew J. Ballard & Joshua Bambrick & Sebastian W. Bodenstein & David , 2024. "Addendum: Accurate structure prediction of biomolecular interactions with AlphaFold 3," Nature, Nature, vol. 636(8042), pages 4-4, December.
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    3. James M. Dewar & Magda Budzowska & Johannes C. Walter, 2015. "The mechanism of DNA replication termination in vertebrates," Nature, Nature, vol. 525(7569), pages 345-350, September.
    4. Devashish Dwivedi & Daniela Harry & Patrick Meraldi, 2023. "Mild replication stress causes premature centriole disengagement via a sub-critical Plk1 activity under the control of ATR-Chk1," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    5. Meng-Fu Bryan Tsou & Tim Stearns, 2006. "Mechanism limiting centrosome duplication to once per cell cycle," Nature, Nature, vol. 442(7105), pages 947-951, August.
    6. Libor Macůrek & Arne Lindqvist & Dan Lim & Michael A. Lampson & Rob Klompmaker & Raimundo Freire & Christophe Clouin & Stephen S. Taylor & Michael B. Yaffe & René H. Medema, 2008. "Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery," Nature, Nature, vol. 455(7209), pages 119-123, September.
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    8. Koki Watanabe & Daisuke Takao & Kei K Ito & Mikiko Takahashi & Daiju Kitagawa, 2019. "The Cep57-pericentrin module organizes PCM expansion and centriole engagement," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
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