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Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis

Author

Listed:
  • Daniel Schator

    (University of California Berkeley)

  • Naren G. Kumar

    (University of California Berkeley)

  • Samuel Joseph U. Chong

    (University of California Berkeley)

  • Timothy K. Jung

    (University of California Berkeley)

  • Eric Jedel

    (University of California Berkeley
    University of California Berkeley)

  • Benjamin E. Smith

    (University of California Berkeley
    University of California Berkeley)

  • David J. Evans

    (University of California Berkeley
    Touro University California)

  • Suzanne M. J. Fleiszig

    (University of California Berkeley
    University of California Berkeley
    University of California Berkeley)

Abstract

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen able to cause life- and sight-threatening infections. Once considered an extracellular pathogen, numerous studies have shown it can survive intracellularly. Previously, we showed that P. aeruginosa inside cells can diversify into distinct subpopulations in vacuoles and the cytoplasm. Here, we report that the transition from vacuoles to cytoplasm requires collaboration with the extracellular subpopulation, through Ca2+ influx enabled by their type III secretion system (T3SS) translocon pore proteins. Moreover, we show that collaboration among P. aeruginosa subpopulations can contribute to disseminating intracellular bacteria in vivo in a mouse infection model. This study lays the groundwork for future investigations into how cooperation between extracellular and intracellular bacteria within the host contributes to disease progression and persistence.

Suggested Citation

  • Daniel Schator & Naren G. Kumar & Samuel Joseph U. Chong & Timothy K. Jung & Eric Jedel & Benjamin E. Smith & David J. Evans & Suzanne M. J. Fleiszig, 2025. "Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62575-3
    DOI: 10.1038/s41467-025-62575-3
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    References listed on IDEAS

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    1. repec:plo:ppat00:1006612 is not listed on IDEAS
    2. Jianfang Li & Matteo M E Metruccio & David J Evans & Suzanne M J Fleiszig, 2017. "Mucosal fluid glycoprotein DMBT1 suppresses twitching motility and virulence of the opportunistic pathogen Pseudomonas aeruginosa," PLOS Pathogens, Public Library of Science, vol. 13(5), pages 1-26, May.
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