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Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli

Author

Listed:
  • Yannick Baffert

    (UMR5086)

  • Nathan Fraikin

    (UMR5086)

  • Yasmine Makhloufi

    (UMR5086)

  • Julie Baltenneck

    (UMR 5240)

  • Marie-Eve Val

    (Département Génomes et Génétique)

  • Annick Dedieu-Berne

    (UMR5086)

  • Jonathan Degosserie

    (CHU UCL Namur)

  • Bogdan I. Iorga

    (Institut de Chimie des Substances Naturelles)

  • Pierre Bogaerts

    (CHU UCL Namur)

  • Erwan Gueguen

    (UMR 5240)

  • Christian Lesterlin

    (UMR5086)

  • Sarah Bigot

    (UMR5086)

Abstract

Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmids, belonging to the IncL group, emerge as the primary vehicle for carbapenem resistance in Enterobacteriaceae. Despite the problematic prevalence of pOXA-48, most research focus on epidemiology and genomics, leaving gaps in our understanding of the mechanisms behind its propagation. In this study, we use a transposon sequencing approach to identify genetic elements critical for plasmid stability, replication, and conjugative transfer. Our results identify a novel type I toxin-antitoxin system, uncharacterized essential maintenance factors, and components of the type IV secretion system and regulatory elements crucial for conjugation. This study advances our understanding of pOXA-48 biology, providing key insights into the genetic factors underlying its successful maintenance and spread in bacterial populations.

Suggested Citation

  • Yannick Baffert & Nathan Fraikin & Yasmine Makhloufi & Julie Baltenneck & Marie-Eve Val & Annick Dedieu-Berne & Jonathan Degosserie & Bogdan I. Iorga & Pierre Bogaerts & Erwan Gueguen & Christian Lest, 2025. "Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli," Nature Communications, Nature, vol. 16(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62404-7
    DOI: 10.1038/s41467-025-62404-7
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