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Vancomycin-resistant enterococci utilise antibiotic-enriched nutrients for intestinal colonisation

Author

Listed:
  • Olivia G. King

    (Imperial College London)

  • Alexander Y. G. Yip

    (Imperial College London)

  • Victoria Horrocks

    (Imperial College London)

  • Jesús Miguéns Blanco

    (Imperial College London)

  • Julian R. Marchesi

    (Imperial College London)

  • Benjamin H. Mullish

    (Imperial College London
    Paddington)

  • Thomas B. Clarke

    (Imperial College London)

  • Julie A. K. McDonald

    (Imperial College London)

Abstract

Antibiotic treatment significantly disrupts the gut microbiome and promotes vancomycin-resistant enterococci (VRE) intestinal colonisation. These disruptions cause the intestine to act as a reservoir for VRE that seed difficult-to-treat infections. Here we show that antibiotics that promote VRE intestinal colonisation increase the concentration of a wide range of nutrients and decrease the concentration of a wide range of microbial metabolites. We show significant but incomplete suppression of VRE growth by individual short chain fatty acids that were decreased in antibiotic-treated faecal microbiomes. However, mixtures of short chain fatty acids provide complete or near complete suppression of VRE growth. We show that VRE use most nutrients increased in antibiotic-treated faecal microbiomes as carbon or nitrogen sources to support their growth, where Enterococcus faecium and Enterococcus faecalis have some common and some distinct preferences for the use of these specific nutrients. Finally, we show that E. faecium and E. faecalis occupy overlapping but distinct nutrient-defined intestinal niches that promote high growth when cultured with each other and when cultured with carbapenem-resistant Enterobacteriaceae. Our results demonstrate that VRE occupy distinct intestinal niches in the antibiotic-treated intestine, defined by their abilities to utilise specific enriched nutrients and their abilities to grow with reduced concentrations of inhibitory microbial metabolites.

Suggested Citation

  • Olivia G. King & Alexander Y. G. Yip & Victoria Horrocks & Jesús Miguéns Blanco & Julian R. Marchesi & Benjamin H. Mullish & Thomas B. Clarke & Julie A. K. McDonald, 2025. "Vancomycin-resistant enterococci utilise antibiotic-enriched nutrients for intestinal colonisation," Nature Communications, Nature, vol. 16(1), pages 1-21, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61731-z
    DOI: 10.1038/s41467-025-61731-z
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    References listed on IDEAS

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    1. Sandrine Isaac & Alejandra Flor-Duro & Gloria Carruana & Leonor Puchades-Carrasco & Anna Quirant & Marina Lopez-Nogueroles & Antonio Pineda-Lucena & Marc Garcia-Garcera & Carles Ubeda, 2022. "Microbiome-mediated fructose depletion restricts murine gut colonization by vancomycin-resistant Enterococcus," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Katharina Brandl & George Plitas & Coralia N. Mihu & Carles Ubeda & Ting Jia & Martin Fleisher & Bernd Schnabl & Ronald P. DeMatteo & Eric G. Pamer, 2008. "Vancomycin-resistant enterococci exploit antibiotic-induced innate immune deficits," Nature, Nature, vol. 455(7214), pages 804-807, October.
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