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Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome

Author

Listed:
  • Christine C. Wu

    (University of Washington)

  • Kristine A. Tsantilas

    (University of Washington)

  • Jea Park

    (University of Washington)

  • Deanna Plubell

    (University of Washington)

  • Justin A. Sanders

    (University of Washington)

  • Previn Naicker

    (CSIR)

  • Ireshyn Govender

    (ReSyn Biosciences)

  • Sindisiwe Buthelezi

    (CSIR)

  • Stoyan Stoychev

    (ReSyn Biosciences)

  • Justin Jordaan

    (ReSyn Biosciences)

  • Gennifer Merrihew

    (University of Washington)

  • Eric Huang

    (University of Washington)

  • Edward D. Parker

    (University of Washington)

  • Michael Riffle

    (University of Washington)

  • Andrew N. Hoofnagle

    (University of Washington)

  • William S. Noble

    (University of Washington
    University of Washington)

  • Kathleen L. Poston

    (Stanford University)

  • Thomas J. Montine

    (Stanford University)

  • Michael J. MacCoss

    (University of Washington)

Abstract

Extracellular vesicles (EVs) in plasma are composed of exosomes, microvesicles, and apoptotic bodies. We report a plasma EV enrichment strategy using magnetic beads called Mag-Net. Proteomic interrogation of this plasma EV fraction enables the detection of proteins that are beyond the dynamic range of liquid chromatography-mass spectrometry of unfractionated plasma. Mag-Net is robust, reproducible, inexpensive, and requires 37,000 peptides from >4,000 proteins. Using Mag-Net on a pilot cohort of patients with neurodegenerative disease and healthy controls, we find 204 proteins that differentiate (q-value

Suggested Citation

  • Christine C. Wu & Kristine A. Tsantilas & Jea Park & Deanna Plubell & Justin A. Sanders & Previn Naicker & Ireshyn Govender & Sindisiwe Buthelezi & Stoyan Stoychev & Justin Jordaan & Gennifer Merrihew, 2025. "Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome," Nature Communications, Nature, vol. 16(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60595-7
    DOI: 10.1038/s41467-025-60595-7
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