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Mechanism of DNA degradation by CBASS Cap5 endonuclease immune effector

Author

Listed:
  • Olga Rechkoblit

    (Icahn School of Medicine at Mount Sinai)

  • Daniela Sciaky

    (Icahn School of Medicine at Mount Sinai)

  • Mi Ni

    (Icahn School of Medicine at Mount Sinai)

  • Yangmei Li

    (Icahn School of Medicine at Mount Sinai)

  • Jithesh Kottur

    (Icahn School of Medicine at Mount Sinai
    Institute of Advanced Virology)

  • Gang Fang

    (Icahn School of Medicine at Mount Sinai)

  • Aneel K. Aggarwal

    (Icahn School of Medicine at Mount Sinai)

Abstract

Bacterial CBASS immune defense systems commonly kill virally infected cells by degrading genomic DNA in a form of cell suicide or abortive infection. We present a high-resolution structure of the CBASS effector Cap5, activated by a cyclic nucleotide, in the act of digesting DNA via tetrameric HNH endonuclease domains. Two HNH domains are in a catalytically active state for cleavage of the DNA strands, whereas the other two HNH domains are in a topologically distinct catalytically inactive state for simply DNA binding. The four HNH domains track one face of the DNA and mark an enzyme that acts as a stand-alone non-specific nuclease. We also show that chromosomally encoded CBASS Cap5 can be extrinsically activated by a cyclic nucleotide, as a step towards potential antibiotics.

Suggested Citation

  • Olga Rechkoblit & Daniela Sciaky & Mi Ni & Yangmei Li & Jithesh Kottur & Gang Fang & Aneel K. Aggarwal, 2025. "Mechanism of DNA degradation by CBASS Cap5 endonuclease immune effector," Nature Communications, Nature, vol. 16(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60484-z
    DOI: 10.1038/s41467-025-60484-z
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