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Structural basis for intrinsic strand displacement activity of mitochondrial DNA polymerase

Author

Listed:
  • Ashok R. Nayak

    (Thomas Jefferson University; 1020 Locust St)

  • Viktoriia Sokolova

    (Thomas Jefferson University; 1020 Locust St)

  • Sirelin Sillamaa

    (Riia 23)

  • Karl Herbine

    (Thomas Jefferson University; 1020 Locust St)

  • Juhan Sedman

    (Riia 23)

  • Dmitry Temiakov

    (Thomas Jefferson University; 1020 Locust St)

Abstract

Members of the Pol A family of DNA polymerases, found across all domains of life, utilize various strategies for DNA strand separation during replication. In higher eukaryotes, mitochondrial DNA polymerase γ relies on the replicative helicase TWINKLE, whereas the yeast ortholog, Mip1, can unwind DNA independently. Using Mip1 as a model, we present a series of high-resolution cryo-EM structures that capture the process of DNA strand displacement. Our data reveal previously unidentified structural elements that facilitate the unwinding of the downstream DNA duplex. Yeast cells harboring Mip1 variants defective in strand displacement exhibit impaired oxidative phosphorylation and loss of mtDNA, corroborating the structural observations. This study provides a molecular basis for the intrinsic strand displacement activity of Mip1 and illuminates the distinct unwinding mechanisms utilized by Pol A family DNA polymerases.

Suggested Citation

  • Ashok R. Nayak & Viktoriia Sokolova & Sirelin Sillamaa & Karl Herbine & Juhan Sedman & Dmitry Temiakov, 2025. "Structural basis for intrinsic strand displacement activity of mitochondrial DNA polymerase," Nature Communications, Nature, vol. 16(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57594-z
    DOI: 10.1038/s41467-025-57594-z
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    References listed on IDEAS

    as
    1. Gina Buchel & Ashok R. Nayak & Karl Herbine & Azadeh Sarfallah & Viktoriia O. Sokolova & Angelica Zamudio-Ochoa & Dmitry Temiakov, 2023. "Structural basis for DNA proofreading," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Tahir H. Tahirov & Dmitry Temiakov & Michael Anikin & Vsevolod Patlan & William T. McAllister & Dmitry G. Vassylyev & Shigeyuki Yokoyama, 2002. "Structure of a T7 RNA polymerase elongation complex at 2.9 Å resolution," Nature, Nature, vol. 420(6911), pages 43-50, November.
    3. Karl Herbine & Ashok R. Nayak & Dmitry Temiakov, 2024. "Structural basis for substrate binding and selection by human mitochondrial RNA polymerase," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
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