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A WYL domain transcription factor regulates Lactiplantibacillus plantarum intestinal colonization via perceiving c-di-GMP

Author

Listed:
  • Quan Guo

    (Hainan University
    Hainan University)

  • Guangqiang Wang

    (University of Shanghai for Science and Technology)

  • Leijie Zheng

    (Hainan University
    Hainan University)

  • Hui Xue

    (Hainan University
    Hainan University)

  • Ruimin Wang

    (Hainan University
    Hainan University)

  • Yajing Fang

    (Hainan University
    Hainan University)

  • Jiachao Zhang

    (Hainan University
    Hainan University)

Abstract

Cyclic diguanosine monophosphate (c-di-GMP) functions as a crucial bacterial second messenger to control diverse biological functions. Although numerous studies have reported the health effects of Lactiplantibacillus plantarum, the regulatory role of c-di-GMP in L. plantarum remains elusive. Here we show that c-di-GMP functions as an important signal molecule for intestinal colonization of L. plantarum. The intracellular c-di-GMP pool in this probiotic is governed principally by the diguanylate cyclases DgcB, DgcC, and DgcD and the phosphodiesterases PdeA and PdeD. Moreover, we reveal that the WYL domain transcription factor MbpR is a c-di-GMP effector in L. plantarum WCFS1. MbpR reduces the transcription level of mucin-binding proteins (MucBPs) via binding to a special motif within the coding sequences. Perception of c-di-GMP by the WYL domain reversed the inhibitory effect of MbpR on the expression of MucBPs, resulting in increased adherence to intestinal epithelial cells by L. plantarum. Overall, our study provides evidence that a WYL domain transcription factor participates in probiotic colonization by sensing c-di-GMP.

Suggested Citation

  • Quan Guo & Guangqiang Wang & Leijie Zheng & Hui Xue & Ruimin Wang & Yajing Fang & Jiachao Zhang, 2025. "A WYL domain transcription factor regulates Lactiplantibacillus plantarum intestinal colonization via perceiving c-di-GMP," Nature Communications, Nature, vol. 16(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57581-4
    DOI: 10.1038/s41467-025-57581-4
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