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In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity

Author

Listed:
  • Ilmatar Rooda

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital
    Tallinn University of Technology)

  • Jasmin Hassan

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital)

  • Jie Hao

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital
    Central South University)

  • Magdalena Wagner

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital)

  • Elisabeth Moussaud-Lamodière

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital)

  • Kersti Jääger

    (University of Tartu
    Competence Centre on Health Technologies)

  • Marjut Otala

    (Helsinki University Hospital, University of Helsinki)

  • Katri Knuus

    (Helsinki University Hospital, University of Helsinki)

  • Cecilia Lindskog

    (Uppsala University)

  • Kiriaki Papaikonomou

    (Karolinska University Hospital
    Karolinska Institutet)

  • Sebastian Gidlöf

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital
    Karolinska Institutet)

  • Cecilia Langenskiöld

    (Sahlgrenska University Hospital)

  • Hartmut Vogt

    (Linköping University)

  • Per Frisk

    (Uppsala University Children’s Hospital)

  • Johan Malmros

    (Karolinska University Hospital
    Karolinska Institutet)

  • Timo Tuuri

    (Helsinki University Hospital, University of Helsinki)

  • Andres Salumets

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital
    Competence Centre on Health Technologies
    University of Tartu)

  • Kirsi Jahnukainen

    (University of Helsinki and Helsinki University Hospital
    Karolinska Institutet and University Hospital)

  • Agne Velthut-Meikas

    (Tallinn University of Technology)

  • Pauliina Damdimopoulou

    (Intervention and Technology, Karolinska Institutet, Huddinge
    Karolinska University Hospital)

Abstract

The ovarian cortical reserve of follicles is vital for fertility. Some medical treatments are toxic to follicles, leading to premature ovarian insufficiency. Ovarian tissue cryopreservation is an established method to preserve fertility in adults and even applied in prepuberty despite unproven efficacy. Here, we analyze transcriptomes of 120 cortical follicles from children and adults for detailed comparison. We discover heterogeneity with two main types of follicles in both age groups: one with expected oocyte-granulosa profiles and another with predicted role in signaling. Transcriptional changes during growth to the secondary stage are similar overall in children and adults, but variations related to extracellular matrix, theca cells, and miRNA profiles are found. Notably, cyclophosphamide dose correlates with interferon signaling in child follicles. Additionally, morphology alone is insufficient for follicle categorization suggesting a need for additional markers. Marker genes for early follicle activation are determined. These findings will help refine follicular classification and fertility preservation techniques across critical ages.

Suggested Citation

  • Ilmatar Rooda & Jasmin Hassan & Jie Hao & Magdalena Wagner & Elisabeth Moussaud-Lamodière & Kersti Jääger & Marjut Otala & Katri Knuus & Cecilia Lindskog & Kiriaki Papaikonomou & Sebastian Gidlöf & Ce, 2024. "In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51185-0
    DOI: 10.1038/s41467-024-51185-0
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