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The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis

Author

Listed:
  • Bangjun Zhou

    (University of Nebraska-Lincoln
    University of Nebraska-Lincoln)

  • Huihui Yu

    (University of Nebraska-Lincoln
    University of Nebraska-Lincoln)

  • Yong Xue

    (University of Nebraska-Lincoln
    University of Nebraska-Lincoln)

  • Mu Li

    (University of Nebraska-Lincoln
    University of Nebraska-Lincoln)

  • Chi Zhang

    (University of Nebraska-Lincoln
    University of Nebraska-Lincoln)

  • Bin Yu

    (University of Nebraska-Lincoln
    University of Nebraska-Lincoln)

Abstract

MicroRNAs (miRNAs) play a key role in regulating gene expression and their biogenesis is precisely controlled through modulating the activity of microprocessor. Here, we report that CWC15, a spliceosome-associated protein, acts as a positive regulator of miRNA biogenesis. CWC15 binds the promoters of genes encoding miRNAs (MIRs), promotes their activity, and increases the occupancy of DNA-dependent RNA polymerases at MIR promoters, suggesting that CWC15 positively regulates the transcription of primary miRNA transcripts (pri-miRNAs). In addition, CWC15 interacts with Serrate (SE) and HYL1, two key components of microprocessor, and is required for efficient pri-miRNA processing and the HYL1-pri-miRNA interaction. Moreover, CWC15 interacts with the 20 S proteasome and PRP4KA, facilitating SE phosphorylation by PRP4KA, and subsequent non-functional SE degradation by the 20 S proteasome. These data reveal that CWC15 ensures optimal miRNA biogenesis by maintaining proper SE levels and by modulating pri-miRNA levels. Taken together, this study uncovers the role of a conserved splicing-related protein in miRNA biogenesis.

Suggested Citation

  • Bangjun Zhou & Huihui Yu & Yong Xue & Mu Li & Chi Zhang & Bin Yu, 2024. "The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46676-z
    DOI: 10.1038/s41467-024-46676-z
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    References listed on IDEAS

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