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Molecular basis promoting centriole triplet microtubule assembly

Author

Listed:
  • Yutaka Takeda

    (The University of Tokyo, Bunkyo)

  • Takumi Chinen

    (The University of Tokyo, Bunkyo)

  • Shunnosuke Honda

    (The University of Tokyo, Bunkyo)

  • Sho Takatori

    (Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo)

  • Shotaro Okuda

    (The University of Tokyo, Bunkyo)

  • Shohei Yamamoto

    (The University of Tokyo, Bunkyo)

  • Masamitsu Fukuyama

    (The University of Tokyo, Bunkyo)

  • Koh Takeuchi

    (Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo)

  • Taisuke Tomita

    (Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo)

  • Shoji Hata

    (The University of Tokyo, Bunkyo)

  • Daiju Kitagawa

    (The University of Tokyo, Bunkyo)

Abstract

The triplet microtubule, a core structure of centrioles crucial for the organization of centrosomes, cilia, and flagella, consists of unclosed incomplete microtubules. The mechanisms of its assembly represent a fundamental open question in biology. Here, we discover that the ciliopathy protein HYLS1 and the β-tubulin isotype TUBB promote centriole triplet microtubule assembly. HYLS1 or a C-terminal tail truncated version of TUBB generates tubulin-based superstructures composed of centriole-like incomplete microtubule chains when overexpressed in human cells. AlphaFold-based structural models and mutagenesis analyses further suggest that the ciliopathy-related residue D211 of HYLS1 physically traps the wobbling C-terminal tail of TUBB, thereby suppressing its inhibitory role in the initiation of the incomplete microtubule assembly. Overall, our findings provide molecular insights into the biogenesis of atypical microtubule architectures conserved for over a billion years.

Suggested Citation

  • Yutaka Takeda & Takumi Chinen & Shunnosuke Honda & Sho Takatori & Shotaro Okuda & Shohei Yamamoto & Masamitsu Fukuyama & Koh Takeuchi & Taisuke Tomita & Shoji Hata & Daiju Kitagawa, 2024. "Molecular basis promoting centriole triplet microtubule assembly," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46454-x
    DOI: 10.1038/s41467-024-46454-x
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