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Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery

Author

Listed:
  • Jinzhong Tian

    (Chinese Academy of Sciences
    Xianghu Laboratory)

  • Wangshuying Deng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Ziwen Zhang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jiaqi Xu

    (ZJU-Hangzhou Global Scientific and Technological Innovation Center)

  • Guiling Yang

    (Xianghu Laboratory)

  • Guoping Zhao

    (Chinese Academy of Sciences)

  • Sheng Yang

    (Chinese Academy of Sciences)

  • Weihong Jiang

    (Chinese Academy of Sciences)

  • Yang Gu

    (Chinese Academy of Sciences)

Abstract

Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium Vibrio natriegens has exceptional FA tolerance and metabolic capacity natively. This bacterium is remodeled by rewiring the serine cycle and the TCA cycle, resulting in a non-native closed loop (S-TCA) which as a powerful metabolic sink, in combination with laboratory evolution, enables rapid emergence of synthetic strains with significantly improved FA-utilizing ability. Further introduction of a foreign indigoidine-forming pathway into the synthetic V. natriegens strain leads to the production of 29.0 g · L−1 indigoidine and consumption of 165.3 g · L−1 formate within 72 h, achieving a formate consumption rate of 2.3 g · L−1 · h−1. This work provides an important microbial chassis as well as design rules to develop industrially viable microorganisms for FA biorefinery.

Suggested Citation

  • Jinzhong Tian & Wangshuying Deng & Ziwen Zhang & Jiaqi Xu & Guiling Yang & Guoping Zhao & Sheng Yang & Weihong Jiang & Yang Gu, 2023. "Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43631-2
    DOI: 10.1038/s41467-023-43631-2
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    References listed on IDEAS

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    1. Ying Xu & Zhe Zhao & Wenhua Tong & Yamei Ding & Bin Liu & Yixin Shi & Jichao Wang & Shenmei Sun & Min Liu & Yuhui Wang & Qingsheng Qi & Mo Xian & Guang Zhao, 2020. "An acid-tolerance response system protecting exponentially growing Escherichia coli," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    2. Deepanwita Banerjee & Thomas Eng & Andrew K. Lau & Yusuke Sasaki & Brenda Wang & Yan Chen & Jan-Philip Prahl & Vasanth R. Singan & Robin A. Herbert & Yuzhong Liu & Deepti Tanjore & Christopher J. Petz, 2020. "Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
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