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Acylation of glycerolipids in mycobacteria

Author

Listed:
  • Shiva Kumar Angala

    (Colorado State University)

  • Ana Carreras-Gonzalez

    (Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas - Universidad del País Vasco/Euskal Herriko Unibertsitatea (CSIC-UPV/EHU)
    Universidad del País Vasco)

  • Emilie Huc-Claustre

    (Colorado State University)

  • Itxaso Anso

    (Cruces University Hospital)

  • Devinder Kaur

    (Colorado State University
    University of Massachusetts Medical School)

  • Victoria Jones

    (Colorado State University)

  • Zuzana Palčeková

    (Colorado State University)

  • Juan M. Belardinelli

    (Colorado State University)

  • Célia Sousa-d’Auria

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Libin Shi

    (Colorado State University)

  • Nawel Slama

    (Institut de Pharmacologie et de Biologie Structurale (IPBS), CNRS, UPS, Université Toulouse III - Paul Sabatier)

  • Christine Houssin

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Annaïk Quémard

    (Institut de Pharmacologie et de Biologie Structurale (IPBS), CNRS, UPS, Université Toulouse III - Paul Sabatier)

  • Michael McNeil

    (Colorado State University)

  • Marcelo E. Guerin

    (Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas - Universidad del País Vasco/Euskal Herriko Unibertsitatea (CSIC-UPV/EHU)
    Universidad del País Vasco
    Cruces University Hospital
    IKERBASQUE, Basque Foundation for Science)

  • Mary Jackson

    (Colorado State University)

Abstract

We report on the existence of two phosphatidic acid biosynthetic pathways in mycobacteria, a classical one wherein the acylation of the sn-1 position of glycerol-3-phosphate (G3P) precedes that of sn-2 and another wherein acylations proceed in the reverse order. Two unique acyltransferases, PlsM and PlsB2, participate in both pathways and hold the key to the unusual positional distribution of acyl chains typifying mycobacterial glycerolipids wherein unsaturated substituents principally esterify position sn-1 and palmitoyl principally occupies position sn-2. While PlsM selectively transfers a palmitoyl chain to the sn-2 position of G3P and sn-1-lysophosphatidic acid (LPA), PlsB2 preferentially transfers a stearoyl or oleoyl chain to the sn-1 position of G3P and an oleyl chain to sn-2-LPA. PlsM is the first example of an sn-2 G3P acyltransferase outside the plant kingdom and PlsB2 the first example of a 2-acyl-G3P acyltransferase. Both enzymes are unique in their ability to catalyze acyl transfer to both G3P and LPA.

Suggested Citation

  • Shiva Kumar Angala & Ana Carreras-Gonzalez & Emilie Huc-Claustre & Itxaso Anso & Devinder Kaur & Victoria Jones & Zuzana Palčeková & Juan M. Belardinelli & Célia Sousa-d’Auria & Libin Shi & Nawel Slam, 2023. "Acylation of glycerolipids in mycobacteria," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42478-x
    DOI: 10.1038/s41467-023-42478-x
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    References listed on IDEAS

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