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HIV-1 diverts cortical actin for particle assembly and release

Author

Listed:
  • Rayane Dibsy

    (University of Montpellier)

  • Erwan Bremaud

    (University of Montpellier)

  • Johnson Mak

    (Griffith University)

  • Cyril Favard

    (University of Montpellier)

  • Delphine Muriaux

    (University of Montpellier)

Abstract

Enveloped viruses assemble and bud from the host cell membranes. Any role of cortical actin in these processes have often been a source of debate. Here, we assessed if cortical actin was involved in HIV-1 assembly in infected CD4 T lymphocytes. Our results show that preventing actin branching not only increases HIV-1 particle release but also the number of individual HIV-1 Gag assembly clusters at the T cell plasma membrane. Indeed, in infected T lymphocytes and in in vitro quantitative model systems, we show that HIV-1 Gag protein prefers areas deficient in F-actin for assembling. Finally, we found that the host factor Arpin, an inhibitor of Arp2/3 branched actin, is recruited at the membrane of infected T cells and it can associate with the viral Gag protein. Altogether, our data show that, for virus assembly and particle release, HIV-1 prefers low density of cortical actin and may favor local actin debranching by subverting Arpin.

Suggested Citation

  • Rayane Dibsy & Erwan Bremaud & Johnson Mak & Cyril Favard & Delphine Muriaux, 2023. "HIV-1 diverts cortical actin for particle assembly and release," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41940-0
    DOI: 10.1038/s41467-023-41940-0
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    References listed on IDEAS

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    1. A. Chaigne & C. Campillo & N. S. Gov & R. Voituriez & C. Sykes & M. H. Verlhac & M. E. Terret, 2015. "A narrow window of cortical tension guides asymmetric spindle positioning in the mouse oocyte," Nature Communications, Nature, vol. 6(1), pages 1-10, May.
    2. Isma Bennabi & Flora Crozet & Elvira Nikalayevich & Agathe Chaigne & Gaëlle Letort & Marion Manil-Ségalen & Clément Campillo & Clotilde Cadart & Alice Othmani & Rafaele Attia & Auguste Genovesio & Mar, 2020. "Artificially decreasing cortical tension generates aneuploidy in mouse oocytes," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    3. Fred E. Fregoso & Trevor Eeuwen & Gleb Simanov & Grzegorz Rebowski & Malgorzata Boczkowska & Austin Zimmet & Alexis M. Gautreau & Roberto Dominguez, 2022. "Molecular mechanism of Arp2/3 complex inhibition by Arpin," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Irene Dang & Roman Gorelik & Carla Sousa-Blin & Emmanuel Derivery & Christophe Guérin & Joern Linkner & Maria Nemethova & Julien G. Dumortier & Florence A. Giger & Tamara A. Chipysheva & Valeria D. Er, 2013. "Inhibitory signalling to the Arp2/3 complex steers cell migration," Nature, Nature, vol. 503(7475), pages 281-284, November.
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