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Functionalization and higher-order organization of liposomes with DNA nanostructures

Author

Listed:
  • Zhao Zhang

    (University of Wisconsin−Madison)

  • Zhaomeng Feng

    (University of Wisconsin−Madison)

  • Xiaowei Zhao

    (Janelia Research Campus, 19700 Helix Drive)

  • Dominique Jean

    (Janelia Research Campus, 19700 Helix Drive)

  • Zhiheng Yu

    (Janelia Research Campus, 19700 Helix Drive)

  • Edwin R. Chapman

    (University of Wisconsin−Madison)

Abstract

Small unilamellar vesicles (SUVs) are indispensable model membranes, organelle mimics, and drug and vaccine carriers. However, the lack of robust techniques to functionalize or organize preformed SUVs limits their applications. Here we use DNA nanostructures to coat, cluster, and pattern sub-100-nm liposomes, generating distance-controlled vesicle networks, strings and dimers, among other configurations. The DNA coating also enables attachment of proteins to liposomes, and temporal control of membrane fusion driven by SNARE protein complexes. Such a convenient and versatile method of engineering premade vesicles both structurally and functionally is highly relevant to bottom-up biology and targeted delivery.

Suggested Citation

  • Zhao Zhang & Zhaomeng Feng & Xiaowei Zhao & Dominique Jean & Zhiheng Yu & Edwin R. Chapman, 2023. "Functionalization and higher-order organization of liposomes with DNA nanostructures," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41013-2
    DOI: 10.1038/s41467-023-41013-2
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    References listed on IDEAS

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    1. Bernard Yurke & Andrew J. Turberfield & Allen P. Mills & Friedrich C. Simmel & Jennifer L. Neumann, 2000. "A DNA-fuelled molecular machine made of DNA," Nature, Nature, vol. 406(6796), pages 605-608, August.
    2. Rasmus P. Thomsen & Mette Galsgaard Malle & Anders Hauge Okholm & Swati Krishnan & Søren S.-R. Bohr & Rasmus Schøler Sørensen & Oliver Ries & Stefan Vogel & Friedrich C. Simmel & Nikos S. Hatzakis & J, 2019. "A large size-selective DNA nanopore with sensing applications," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    3. Henri G. Franquelim & Alena Khmelinskaia & Jean-Philippe Sobczak & Hendrik Dietz & Petra Schwille, 2018. "Membrane sculpting by curved DNA origami scaffolds," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    4. Shawn M. Douglas & Hendrik Dietz & Tim Liedl & Björn Högberg & Franziska Graf & William M. Shih, 2009. "Self-assembly of DNA into nanoscale three-dimensional shapes," Nature, Nature, vol. 459(7245), pages 414-418, May.
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