IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-40146-8.html
   My bibliography  Save this article

Energetic robustness to large scale structural fluctuations in a photosynthetic supercomplex

Author

Listed:
  • Dvir Harris

    (Massachusetts Institute of Technology)

  • Hila Toporik

    (Arizona State University
    Arizona State University
    The Hebrew University of Jerusalem)

  • Gabriela S. Schlau-Cohen

    (Massachusetts Institute of Technology)

  • Yuval Mazor

    (Arizona State University
    Arizona State University)

Abstract

Photosynthetic organisms transport and convert solar energy with near-unity quantum efficiency using large protein supercomplexes held in flexible membranes. The individual proteins position chlorophylls to tight tolerances considered critical for fast and efficient energy transfer. The variability in protein organization within the supercomplexes, and how efficiency is maintained despite variability, had been unresolved. Here, we report on structural heterogeneity in the 2-MDa cyanobacterial PSI-IsiA photosynthetic supercomplex observed using Cryo-EM, revealing large-scale variances in the positions of IsiA relative to PSI. Single-molecule measurements found efficient IsiA-to-PSI energy transfer across all conformations, along with signatures of transiently decoupled IsiA. Structure based calculations showed that rapid IsiA-to-PSI energy transfer is always maintained, and even increases by three-fold in rare conformations via IsiA-specific chls. We postulate that antennae design mitigates structural fluctuations, providing a mechanism for robust energy transfer in the flexible membrane.

Suggested Citation

  • Dvir Harris & Hila Toporik & Gabriela S. Schlau-Cohen & Yuval Mazor, 2023. "Energetic robustness to large scale structural fluctuations in a photosynthetic supercomplex," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40146-8
    DOI: 10.1038/s41467-023-40146-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-40146-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-40146-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Hila Toporik & Anton Khmelnitskiy & Zachary Dobson & Reece Riddle & Dewight Williams & Su Lin & Ryszard Jankowiak & Yuval Mazor, 2020. "Author Correction: The structure of a red-shifted photosystem I reveals a red site in the core antenna," Nature Communications, Nature, vol. 11(1), pages 1-1, December.
    2. E. J. Boekema & A. Hifney & A. E. Yakushevska & M. Piotrowski & W. Keegstra & S. Berry & K.-P. Michel & E. K. Pistorius & J. Kruip, 2001. "A giant chlorophyll–protein complex induced by iron deficiency in cyanobacteria," Nature, Nature, vol. 412(6848), pages 745-748, August.
    3. Lars V. Bock & Helmut Grubmüller, 2022. "Effects of cryo-EM cooling on structural ensembles," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Hila Toporik & Anton Khmelnitskiy & Zachary Dobson & Reece Riddle & Dewight Williams & Su Lin & Ryszard Jankowiak & Yuval Mazor, 2020. "The structure of a red-shifted photosystem I reveals a red site in the core antenna," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jianying Zhu & Qi Zhang & Hui Zhang & Zuoqiang Shi & Mingxu Hu & Chenglong Bao, 2023. "A minority of final stacks yields superior amplitude in single-particle cryo-EM," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Ryo Nagao & Koji Kato & Tasuku Hamaguchi & Yoshifumi Ueno & Naoki Tsuboshita & Shota Shimizu & Miyu Furutani & Shigeki Ehira & Yoshiki Nakajima & Keisuke Kawakami & Takehiro Suzuki & Naoshi Dohmae & S, 2023. "Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Nermin A. El Semary, 2022. "Iron-Marine Algal Interactions and Impacts: Decreasing Global Warming by Increasing Algal Biomass," Sustainability, MDPI, vol. 14(16), pages 1-11, August.
    4. Pedram Mehrabi & Sihyun Sung & David Stetten & Andreas Prester & Caitlin E. Hatton & Stephan Kleine-Döpke & Alexander Berkes & Gargi Gore & Jan-Philipp Leimkohl & Hendrik Schikora & Martin Kollewe & H, 2023. "Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40146-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.