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Spatially controlled construction of assembloids using bioprinting

Author

Listed:
  • Julien G. Roth

    (Stanford University School of Medicine
    Stanford University
    Safety Assessment, Genentech Inc.)

  • Lucia G. Brunel

    (Stanford University)

  • Michelle S. Huang

    (Stanford University)

  • Yueming Liu

    (Stanford University)

  • Betty Cai

    (Stanford University)

  • Sauradeep Sinha

    (Stanford University)

  • Fan Yang

    (Stanford University
    Stanford University School of Medicine)

  • Sergiu P. Pașca

    (Stanford University
    Stanford University)

  • Sungchul Shin

    (Stanford University)

  • Sarah C. Heilshorn

    (Stanford University
    Stanford University)

Abstract

The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to organoids with conserved cytoarchitectures that are susceptible to plastic deformation. Here, we develop a platform, termed Spatially Patterned Organoid Transfer (SPOT), consisting of an iron-oxide nanoparticle laden hydrogel and magnetized 3D printer to enable the controlled lifting, transport, and deposition of organoids. We identify cellulose nanofibers as both an ideal biomaterial for encasing organoids with magnetic nanoparticles and a shear-thinning, self-healing support hydrogel for maintaining the spatial positioning of organoids to facilitate the generation of assembloids. We leverage SPOT to create precisely arranged assembloids composed of human pluripotent stem cell-derived neural organoids and patient-derived glioma organoids. In doing so, we demonstrate the potential for the SPOT platform to construct assembloids which recapitulate key developmental processes and disease etiologies.

Suggested Citation

  • Julien G. Roth & Lucia G. Brunel & Michelle S. Huang & Yueming Liu & Betty Cai & Sauradeep Sinha & Fan Yang & Sergiu P. Pașca & Sungchul Shin & Sarah C. Heilshorn, 2023. "Spatially controlled construction of assembloids using bioprinting," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40006-5
    DOI: 10.1038/s41467-023-40006-5
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    References listed on IDEAS

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    1. Fikri Birey & Jimena Andersen & Christopher D. Makinson & Saiful Islam & Wu Wei & Nina Huber & H. Christina Fan & Kimberly R. Cordes Metzler & Georgia Panagiotakos & Nicholas Thom & Nancy A. O’Rourke , 2017. "Assembly of functionally integrated human forebrain spheroids," Nature, Nature, vol. 545(7652), pages 54-59, May.
    2. Ashot S. Harutyunyan & Brian Krug & Haifen Chen & Simon Papillon-Cavanagh & Michele Zeinieh & Nicolas De Jay & Shriya Deshmukh & Carol C. L. Chen & Jad Belle & Leonie G. Mikael & Dylan M. Marchione & , 2019. "H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    3. Andrew C. Daly & Matthew D. Davidson & Jason A. Burdick, 2021. "3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
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